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Hambaryan L. R. et al.: Peculiarities of Development of Phytoplankton as an Indicator of the Ecological State in
Modern Hydrochemical Conditions of Masrik River
of waters, it should be aware that the origins of the river
forms within the biochemical province, where in different
substrates is present natural higher contents of the individual
components, which means high natural geochemical
background, due to the influence of ophiolite rocks [9,10].
2.1. Sampling Methods
Before collecting of each sample, bottles were washed 3-4
times with testing water. The level of the bottled water
samples met the requirements of samples selectionmaximum. Collection, preservation and processing of algae
were conducted according to standard methodology adopted
in hydrobiology [3].
2.2. Chemical Analysis Methods
the analysis of water samples.
Different determinants were used for clarifying the species
of algae [4, 5, 6, 7]. Quantitative analysis of phytoplankton
samples produced in a Nageotte chamber (V=0.1 ml). The
Nageotte chamber filled 7 times. Simultaneously with the
determination of quantity, the measurement of cell volume of
massive forms of phytoplankton was performed.
The Nageotte chamber filled 7 times. Simultaneously with
the determination of quantity, the measurement of cell
volume of massive forms of phytoplankton was performed.
The biomass was identified by individual volume of the
cells for each type of phytoplankton in the sample. Relative
weight of algae considered as 1.
The biomass of i species are calculated using following
formula.
The table 1 shows the list of methods that were used for
Table 1. The methods for Determination of Different Elements and Compounds in Water
N
Elements and Compounds
Methods of Determination
Sensitivity of Analysis
Method
Links
1
Na+
Flame-photometric analysis
0.1
ISO 9964-3
2
K+
Flame-photometric analysis
0.3
ISO 9961-3
2+
3
Ca
4
Mg2+
Volumetric-trilonometric analysis
ISO 6058
Volumetric-trilonometric analysis
5
-
ISO 7980
6
Cl
Volumetric method with silver nitrate
ISO 9297
SO42-
Weights methods
7
PO4
3-
ISO 9280
8
NO3-
9
NO2
-
10
Ni+
11
12
13
Colorimetric method with ammonium molybdate
0.01
ISO 6878
Colorimetric method with sodium salicilate
0.2
ISO 7890-3
Colorimetric method with Griess reagent
0.01
ÃÎÑÒ 4192-82
Colorimetric method with dimethylglixime
0.005
ISO 8288
Cr
Colorimetric method with diphenylcloride
0.005
ISO 11083
Cu2+
Polarographic method
0.0005
Instrument manual
2+
Polarographic method
0.00005
Instrument manual
Pb
Вi =NiVi,
where
Bi-the biomass of i species, Ni-its quantity, Vi-average
volume of the cell.
For assessment the level of contamination of Masrik river
water saprobien system of Kolkwitz and Marson were used,
particularly modified by Zelinka and Marvana. For
calculation of saprobioty steps, the list of algae were usedindicators of saprobioty [8].
3. Results and Discussions
3.1. Hydrochemical Study of Masrik River Water
According to hydrochemical research of water, the pH
ranged from 6.5-7.3. Compared with the spring of the
previous year in 2014, pH was lower. Total mineralization
varied within the limits of 60-430 mg/L, and water hardness
averaged 0,6-5,6 mg-eqv/L. Moreover, it should be noted that
the water of the river Sotk had higher salinity and hardness
(3-5,6 mg-eqv/L), and according to the classification
described as moderately hard. According to its ionic
composition, the water of Sotk river can be classified as a
hydrocarbonate- calcium-magnesium, and the water of
Masrik river - hydrocarbonate-sulphate-calcium-magnesiumsodium.
In 2014, BOD5 in different parts of watercourses of the
river amounted respectively - 10.8 (at upper- Sotk inflow),
4.2 (on average) and 4.5 (lower) parts of the river Masrik,
indicating a relatively high level of organic contamination of
the waters of the river.
Modern research and evaluation of surface water quality,
that are presented in the article are based on national
standards adopted in 2011 and developed with point of view
of the basin management of the river watershed. According to
the assessment of the content of major ions, nutrients and
heavy metals in the water, by the norms of surface water
quality and by taking into account geoch