Phytoplankton of river Masrik | Page 3

46 Hambaryan L. R. et al.: Peculiarities of Development of Phytoplankton as an Indicator of the Ecological State in Modern Hydrochemical Conditions of Masrik River of waters, it should be aware that the origins of the river forms within the biochemical province, where in different substrates is present natural higher contents of the individual components, which means high natural geochemical background, due to the influence of ophiolite rocks [9,10]. 2.1. Sampling Methods Before collecting of each sample, bottles were washed 3-4 times with testing water. The level of the bottled water samples met the requirements of samples selectionmaximum. Collection, preservation and processing of algae were conducted according to standard methodology adopted in hydrobiology [3]. 2.2. Chemical Analysis Methods the analysis of water samples. Different determinants were used for clarifying the species of algae [4, 5, 6, 7]. Quantitative analysis of phytoplankton samples produced in a Nageotte chamber (V=0.1 ml). The Nageotte chamber filled 7 times. Simultaneously with the determination of quantity, the measurement of cell volume of massive forms of phytoplankton was performed. The Nageotte chamber filled 7 times. Simultaneously with the determination of quantity, the measurement of cell volume of massive forms of phytoplankton was performed. The biomass was identified by individual volume of the cells for each type of phytoplankton in the sample. Relative weight of algae considered as 1. The biomass of i species are calculated using following formula. The table 1 shows the list of methods that were used for Table 1. The methods for Determination of Different Elements and Compounds in Water N Elements and Compounds Methods of Determination Sensitivity of Analysis Method Links 1 Na+ Flame-photometric analysis 0.1 ISO 9964-3 2 K+ Flame-photometric analysis 0.3 ISO 9961-3 2+ 3 Ca 4 Mg2+ Volumetric-trilonometric analysis ISO 6058 Volumetric-trilonometric analysis 5 - ISO 7980 6 Cl Volumetric method with silver nitrate ISO 9297 SO42- Weights methods 7 PO4 3- ISO 9280 8 NO3- 9 NO2 - 10 Ni+ 11 12 13 Colorimetric method with ammonium molybdate 0.01 ISO 6878 Colorimetric method with sodium salicilate 0.2 ISO 7890-3 Colorimetric method with Griess reagent 0.01 ÃÎÑÒ 4192-82 Colorimetric method with dimethylglixime 0.005 ISO 8288 Cr Colorimetric method with diphenylcloride 0.005 ISO 11083 Cu2+ Polarographic method 0.0005 Instrument manual 2+ Polarographic method 0.00005 Instrument manual Pb Вi =NiVi, where Bi-the biomass of i species, Ni-its quantity, Vi-average volume of the cell. For assessment the level of contamination of Masrik river water saprobien system of Kolkwitz and Marson were used, particularly modified by Zelinka and Marvana. For calculation of saprobioty steps, the list of algae were usedindicators of saprobioty [8]. 3. Results and Discussions 3.1. Hydrochemical Study of Masrik River Water According to hydrochemical research of water, the pH ranged from 6.5-7.3. Compared with the spring of the previous year in 2014, pH was lower. Total mineralization varied within the limits of 60-430 mg/L, and water hardness averaged 0,6-5,6 mg-eqv/L. Moreover, it should be noted that the water of the river Sotk had higher salinity and hardness (3-5,6 mg-eqv/L), and according to the classification described as moderately hard. According to its ionic composition, the water of Sotk river can be classified as a hydrocarbonate- calcium-magnesium, and the water of Masrik river - hydrocarbonate-sulphate-calcium-magnesiumsodium. In 2014, BOD5 in different parts of watercourses of the river amounted respectively - 10.8 (at upper- Sotk inflow), 4.2 (on average) and 4.5 (lower) parts of the river Masrik, indicating a relatively high level of organic contamination of the waters of the river. Modern research and evaluation of surface water quality, that are presented in the article are based on national standards adopted in 2011 and developed with point of view of the basin management of the river watershed. According to the assessment of the content of major ions, nutrients and heavy metals in the water, by the norms of surface water quality and by taking into account geoch