WAVEFRONT CONTROL
FOCUS
Figure 4. Principle of Single Molecule Localization Microscopy.( a) A super-resolved image of an object is created by taking thousands of images, each showing different small subsets of the entity of fluorophores.( b) Experimental SMLM image showing nuclear pores inside the nuclear membrane of a U2OS cell. The colour hue indicates the axial coordinate. The inset shows a few magnified nuclear pore complexes.( c) NPC reconstruction from simulated, aberrated SMLM data: While digital AO is able to increase the number of successful single molecule fits and remove systematic errors, it is unable to increase the signal-to-noise ratio, hence unable to improve the localization precision. The figure is adapted from [ 6 ] under the CC BY 4.0 license.
REFERENCES
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[ 2 ] F. Helmchen, W. Denk, Nature Methods 2( 12), 932( 2005)
[ 3 ] M. A. May, N. Barré, K. Kummer, M. Kress, M. Ritsch-Marte, A. Jesacher, Nat. Commun. 12( 1), 4340( 2021)
[ 4 ] J. D. Muñoz-Bolaños, P. Rajaeipour, K. Kummer, M. Kress, M., Ç. Ataman, M. Ritsch-Marte, A. Jesacher, ACS Photonics 12( 1), 176( 2025)
[ 5 ] M. Lelek et al., Nat. Rev. Methods Primers 1, 39( 2021)
[ 6 ] J. G. Maloberti, L. Velas, S. Moser, A. Gaugutz, M. Bishara, G. J. Schütz, A. Jesacher, Biomed. Opt. Express 16( 4), 1310( 2025)
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