Phalaenopsis Journal First Quarter 2014 | Page 25

ers, such as orchids, could not have arisen by chance. Darwin viewed floral diversification as evidence for his theory of evolution by natural selection. His works have provided the conceptual underpinning for understanding that floral adaptations are the mechanisms responsible for evolutionary transitions in reproductive systems. Olof Swartz, a Swedish botanist, who studied under Linnaeus filius, was the first specialist in orchid taxonomy; he published a morphological analysis and a classification in modern orchid terminology (1797). Louis Claude Richard (1817) a French botanist, gave us much of the descriptive terminology for sexual morphology used in orchid literature in a paper by on European orchids. Richard emphasized the structure of the pollinia and details of the gynostemium and depicted details of the critical characters on a Genitalia Orchidearum plate. John Lindley, English botanist, expanded hierarchical orchid classification by using a category between family and tribe in his Orchidearum Sceletos (1826) that has become subfamily. Lindley became convinced of the superiority of the “natural” classification system of Jussieu over the “artificial” system of Linnaeus through arduous study of character patterns. George Bentham continued Lindley’s work in collaboration with Sir Joseph Hooker. Their great systematic work for flowering plants, Genera Plantarum (1883), became the classification system used in the Kew Herbarium. The German biologist Willi Hennig is considered the founder of phylogenetic systematics (cladistics). His approach to biological classification is to group organisms based on whether or not they have one or more shared unique characteristics that come from the group’s last common ancestor. Members of that group are thought to share a common history and therefore are considered to be more closely related. Hennig’s book, Phylogenetic Systematics (1966), introduced the cladistic analysis methods that would be critical in the coming age of molecular systematics. Robert Dressler’s world-class treatment of the family, The orchids: Natural History and Classification (1981), was heralded as a modern multidisciplinary treatment of Orchidaceae that would be a significant factor for next generation of orchidologists. Dressler updated his treatment with the truly superb Phylogeny and Classification of the Orchid Family (1993). As good as this comprehensive classification is, it relies heavily on morphology and a few characters, such as anther configuration and pollinarium structure and therefore some of the taxa are not monophyletic. Deoxyribonucleic acid (DNA) may be considered the ultimate form of sexual expression. By the late 1940s, DNA was largely accepted as the genetic molecule. Watson and Crick showed that each strand of the DNA molecule was a template for the other strand (1953). Their discovery has been called the most important biological find of the last 100 years, and the prediction that it opened the scientific frontier for the next 100 years is coming to fruition. The polymerase chain reaction (PCR), developed in 1983 by Kary Mullis, was a biochemical technology needed to sequence DNA. The indispensable technique allows a single piece of DNA to be amplified generating thousands to millions of copies. These copies are used for DNA sequencing, combined with computer analysis to create a DNA-based phylogeny. The groundwork for Genera Orchidacearum started with traditional medicine, preceded into artificial grouping, advanced to natural classifications, included expanded character sets, added new methods of analyses, and became