PGLO Lab Jan. 2013 | Page 7

DIY

make your own transformation solution!

HOW TO

7

INTRODUCING

make your own

transformation solution!

To make a home-made CaCl2 transformation solution, similar to a store-bought one, you will need to know the following values:

CaCl2.2H2O

100mL

50mM

pH 6.1

Calculate the mass required of CaCl2.2H20 powder by following this formula:

If done correctly it should look something like this:

Place a 250mL beaker on a scale, zero the scale, and weigh out 0.74g of CaCl2.2H20 into the beaker. Then, fill the beaker with purified or distilled water to the 100mL mark. Stir to mix.

Your transformation solution is now ready to use!

INTRODUCING

INTRODUCING

INTRODUCING

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