PGLO Lab Jan. 2013 | Page 15

WHY THESE RESULTS, YOU ASK?

The results produced suggest a major error occured during the laoratory procedure. The procedure was to yield petri dishes filled with successfully transformed bacteria. This was to be done by transferring the bacteria onto the mediums in the petri dishes using an inoculation loop. Though, in order to transfer the bacteria the loop cannot be hot. If the loop is hot, the bacteria will die, resulting in a failure to grow bacteria that glow under the UV light, which results from the pGLO plasmid and protein expression.

Transformation efficiency is a calculation that determines how efficient the values used within the procedure were, overall, and how efficient the scientists were at getting the pGLO plasmid into a bacterium.