5. Add 5 milliliters of dichloromethane to the separatory funnel. Stopper the funnel and grab the neck of the funnel with one hand, holding the stopper into the neck of the funnel. With your other hand grasp the stopcock in such a way that you can turn the plug in the barrel to open and close the stopcock. While holding the stopper tightly into the neck of the funnel, invert the funnel so the liquid no longer is in contact with the stopcock. Open the stopcock to release any pressure that may have built up inside the funnel. Close the stopcock and agitate the material in the funnel without shaking it. Point the stem of the funnel upward, and making sure no liquid is in contact with the stopcock open it, to again relieve any pressure build-up. Close the stopcock. Agitate again as before and reclamp the separatory funnel to the ring stand.
PART II
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