NTU Undergraduates' research April 2014 - Biosciences | Page 31

Emma Jones
Cyclin B1: Biological Interaction Network in Breast Cancer

ABSTRACT

Cyclin B1 (CCNB1) is a crucial member in the orchestration of the cell cycle through its regulatory
role in the G2-M stage. Such pathways are shown to undergo dysfunction in cancerous states.
Research into CCNB1 involvement in breast cancer mainly consists of the genes prognostic value and
there is little data on the possible linkages between the gene and other biomolecules involved in its
biological interaction system within the disease. This study unpinned some of those biological
interaction networks using systems biology based methodology. The application of Artificial Neural
Network (ANN) which employed the back propagation algorithm was undertaken and determined
the hypothetical relationships between the genes in the proposed CCNB1interaction network. ANN
predicted the genes that are strongly associated to CCNB1 within breast cancer tissues; which
included genes like CDK1 and NUSAP1. Also analysis of eight predicted associations of CCNB1 in
normal tissues was applied as further ANN input genes, which produced a common gene signature
of six genes within the breast cancer dataset; the six genes interlinked CCNB1 in a variety of ways
with the eight gene targets. The most prevalent being KPNA2, which along with the other mutual
genes produced are associated with a variety of cancers, therefore the resulting gene signature may
benefit in the construction of novel therapeutics that could tackle the disease.
Emma Jones