NTU Undergraduates' research April 2014 - Biosciences | Page 143

RESEARCH PAPER

Effect of Naringin flavonoid
on H9c2 cell line
C. Pleguezuelos Manzano
Nottingham Trent University, School of Science and Technology,
Nottingham, England, United Kingdom

BACKGROUND AND PURPOSE
Naringin is a flavonoid that is present naturally in a broad range of citrus fruits but particularly in grapefruits. Flavonoids
play an important role in animal organisms as they are antioxidant molecules and have beneficial properties regarding
cardiovascular diseases. Reactive oxygen species are the main molecules involved in the appearance of cardiac injuries
and cardiac diseases, particularly H2O2. The possible cardio-protective and/or cytotoxic effect of Naringin on
cardiomyoblast (H9c2) cell culture.
EXPERIMENTAL APPROACH
Cell viability of cardiomyoblast cells and cell differentiated to cardiomyocytes, under different naringin dose-response
treatments was assessed using the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay. Also
the cardio-protective effect of the flavonoid was tested against the oxidative stress induced by H 2O2 (800 µM).
KEY RESULTS
Naringin results in non-cytotoxicity at any concentration tested in both non-differentiated and differentiated cells. In
addition, naringin (100 µM) showed cardio-protective effects on the cells cultivated in the presence of H2O2 (800 µM).
CONCLUSION AND IMPLICATIONS
The cardio-protective action together with the no cytotoxic effect of naringin on the H9c2 culture makes naringin a
good candidate for the development of drugs and treatments against oxidative stress induced injuries in cardiac tissues.
Further investigations are required to clarify the molecular mechanisms involved in the naringin action.