NTU Undergraduates' research April 2014 - Biosciences | 页面 106

Abstract

BACKGROUND AND PURPOSE: H9c2 cells extracted from rat ventricles have the potential to
differentiate into cardiac or skeletal muscle. The cell line is therefore a useful model to
investigate the cardiotoxic and cardio protective properties of various agents.
Myricetin (3, 3’ ,4’ , 5, 5’ , 7- hexahydroxyflavone) is a naturally occurring flavonol that is found in
tea, grapes and cranberries. The position, number and substitution of hydroxyl groups on the B
ring and the saturation of the C2-C3 of myricetin has been found to contribute to its antioxidant
and pro-oxidant effects in differentiated and undifferentiated H9c2 cells.
EXPERIMENTAL APPROACH: Cell viability was estimated using the MTT T (3-(4, 5dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assay. Coumassie Brilliant Blue staining
was carried out to observe the morphological changes in myricetin treated cells.
KEY RESULTS: A dose-dependent increase in apoptosis was seen in the differentiated and
undifferentiated cells; with higher concentrations of myricetin causing the most cytotoxic effect.
Myricetin was also found to have cardio protective effects on oxidative stress induced
undifferentiated cells.
CONCLUSIONS AND IMPLICATIONS: Myricetin was found to be a potent inducer of cellular
apoptosis at long incubation periods . The flavonol employs numerous mechanisms to
bring this about e.g. inhibition of enzymes that regulate normal DNA replication
(topoisomerases and polymerases). It was also found to be cardio protective against
oxidative stress. Which has vast applications in the treatment major cardiovascular
diseases.