NEXT GENERATION GENOME EDITING TECHNOLOGIES Next-Generation-Genome-Editing-Technologies | Page 4

INTRODUCTION
Renaissance took a lot of pain to take over the dark age of knowledge. Man was unaware of the
living beings and varieties of species surrounding him. Nobody in that era would have thought
that a day would come when evolution will not seem magical, instead man himself would
become capable of instigating evolution. It is said that true powers lie in bringing out a change
that we want to see and it is true philosophically as well as genetically. Genetically modified
organisms had still not phased out of minds, when hype got created about how these organisms
are made. Curiosity lies in the methods devised for genetic modification. Though, there are many
methods to do so, but one has made itself stuck in bright minds of researchers.
Source: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0015082
Genome editing with engineered nucleases, which can be designed to specifically target a
sequence of DNA has been exploited since 1990, when Zinc finger nucleases came into light.
Using the property of nucleases along with exploiting endogenous DNA repair apparatus, the
DNA of higher forms could be altered with accuracy.