My first work Brief Introduction of Transmission Electron Micros
Brief Introduction of Transmission Electron Microscopy
Transmission Electron Microscope (TEM), can see in the optical Microscope can not see less
than 0.2um fine structure, these structures are called submicroscopic structure or
ultrastructure.To see these structures clearly, a shorter wavelength light source must be
selected to improve the resolution of the microscope.In 1932, Ruska invented the
transmission electron microscope using electron beam as the light source. The wavelength of
electron beam is much shorter than that of visible and ultraviolet light, and the wavelength
of electron beam is inversely proportional to the square root of the voltage of emitting
electron beam. TEM resolution is up to 0.2nm.
Electron microscope and optical microscope are basically the same imaging principle, except
that the former USES electron beam as the light source and electric magnetic field as the
lens.In addition, due to the weak penetration of electron beam, specimens used in electron
microscopy must be made into ultrathin sections with a thickness of about 50nm.These slices
are made with ultramicro slicers. Electron microscope magnification of up to nearly ten
thousand times, by the lighting system, imaging system, vacuum system, recording system,
power system five parts, if segmentation: the body is the electron lens and video recording
system, by the vacuum electron gun, condenser, content of sample room, objective, the
diffraction lens, lens, projector lens, the screen and a camera in the middle.
Electron microscope is a microscope that USES electrons to show the interior or surface of an
object.The wavelength of the high-speed electron is shorter than that of visible light
(wave-particle duality), and the resolution of the microscope is limited by the wavelength it