18
LMS Issue 4 | 2014
Biotechnology & Pharmaceuticals
Three Lasers
Flow cytometry is a technology used to measure and
analyse the properties of cells as they flow in a stream of
liquid through a laser. This technology is used to perform
several procedures, including cell counting, biomarker
detection, protein engineering and cell sorting. A flow
cytometer allows one to analyse thousands of particles in
real time, with the ability to separate specific particles.
T
he g uava easy Cy te™ 12 f low
cytometer uses three lasers (488,
642 and 405nm) to achieve up to 12
simultaneous detection parameters,
including 10 fluorescent colours plus
forward and side scatter. In the two and
three laser systems, the lasers overlap
spatially and are modulated out of phase
at high frequency so that each particle
is sampled many times as it travels
through the overlapped beams. Modulation is particularly important for
identifying dyes, which have overlapping emissions such PE-Cy7 (blue laser
excitation) and APC-Cy7 (red laser
excitation). Unlike spatially separated
beams, modulation eliminates the need
for time-delay calibration, simplifying
the overall operation of the instrument.
Their micro-capillary flow cytometry
systems are simpler to operate and easier
to maintain. They utilise small sample
volumes, generate minimal waste
and have lower operating costs. Thus,
guava easyCyte™ flow cytometers are
uniquely amenable to on-demand use
in the laboratory and have helped many
scientists achieve insightful cellular
analysis since 1998.These cytometers
provide the analytical power needed to
expand research horizons.
The easyCyte family also meets
sample throughput needs by offering
both single sample and multi-sample
processing. The guava easyCyte HT
instruments provide high throughput
analysis with a robotic sample tray
that automatically handles a 96-well
microplate and up to 10 sample tubes. ⚛
Merck Millipore
Field Marketing Manager: Adele Heath
Tel: 011-372-5129
Email: [email protected]