LMS Volume 33 |ssue 4 | Page 18

18  LMS  Issue 4 | 2014 Biotechnology & Pharmaceuticals Three Lasers Flow cytometry is a technology used to measure and analyse the properties of cells as they flow in a stream of liquid through a laser. This technology is used to perform several procedures, including cell counting, biomarker detection, protein engineering and cell sorting. A flow cytometer allows one to analyse thousands of particles in real time, with the ability to separate specific particles. T he g uava easy Cy te™ 12 f low cytometer uses three lasers (488, 642 and 405nm) to achieve up to 12 simultaneous detection parameters, including 10 fluorescent colours plus forward and side scatter. In the two and three laser systems, the lasers overlap spatially and are modulated out of phase at high frequency so that each particle is sampled many times as it travels through the overlapped beams. Modulation is particularly important for identifying dyes, which have overlapping emissions such PE-Cy7 (blue laser excitation) and APC-Cy7 (red laser excitation). Unlike spatially separated beams, modulation eliminates the need for time-delay calibration, simplifying the overall operation of the instrument. Their micro-capillary flow cytometry systems are simpler to operate and easier to maintain. They utilise small sample volumes, generate minimal waste and have lower operating costs. Thus, guava easyCyte™ flow cytometers are uniquely amenable to on-demand use in the laboratory and have helped many scientists achieve insightful cellular analysis since 1998.These cytometers provide the analytical power needed to expand research horizons. The easyCyte family also meets sample throughput needs by offering both single sample and multi-sample processing. The guava easyCyte HT instruments provide high throughput analysis with a robotic sample tray that automatically handles a 96-well microplate and up to 10 sample tubes. ⚛ Merck Millipore Field Marketing Manager: Adele Heath Tel: 011-372-5129 Email: [email protected]