36
Molecular & Cell Biology
LMS Issue 3 | 2014
Cell Culture by Numbers
62
Years (1952) since the first
growth factor, nerve growth
factor, was discovered by Rita LeviMontalicini. Find Gibco® growth factors
at lifetechnologies.com/growthfactors
Of f low c y tomet r y
researchers start with
cell culture
92%
Thermo Fisher Scientific
Contact: Candice Johnston
Tel:
+27(0)83 293 7113
Email: [email protected]
22
The number of Gibco DMEM
formulations. Find the right
formulation for one’s cells at lifetechnologies.com/dmem
Years Gibco has been providing cell culture researchers
with premium cell culture products
Of humidity required
to prevent media
from evaporating and compromising cells. See lifetechnologies.com/
cellculturebasics
Days to get pluripotent stem
cells to transform to neural cells
using neural induction medium. Visit
lifetechnologies.com/nscdiff
52
95%
7
8
The number of components in
Essential 8™ Medium, which is
all that is needed to culture pluripotent
stem cells. See lifetechnologies.com/
essential8
Years since the launch of the
B-27®. Get the B-27® product
that ensures cell line happiness at
lifetechnologies.com/b27
From the basic formulations to the
newest innovations, Life Technologies™
Gibco products provide the highest
quality, consistency and performance
for cell culture and tissue culture needs
including cell culture media, reagents,
cell lines, cell cultures, cell culture bags,
21
and growth factors for research and
bioproduction needs. Find out more at
lifetechnologies.com/gibco ⚛
State-of-the-art Centrifuges
T
he newest generation of SIGMA
laboratory centrifuges (like the
high-speed desktop centrifuge 1-14) are
equipped with state-of-the-art electronics. They are driven by brushless, silent
and long-life asynchronous motors. The
problem of carbon brush change is
no longer existent and as there is
no carbon dust pollution, operation in cleanrooms is possible
if the appropriate accessories
are used. Their high-speed
desktop centrifuge 1-14 is
ideally suited for sedimentation in 0.2ml through
2.2ml micro-tubes. The
large display allows high
legibility of the values for speed, relative
centrifugal force and running time. The
setting of these parameters is also quick
and simple with push buttons.
The SIGMA 1-14 features:
• Bright LCD display, which offers
good legibility
• Large capacity 24 x 1.5 – 2.2ml
• Top speed 14.800rpm = 16.163 x g/
min. It also has a very high relative
centrifugal force of 2 – 16.163 x g.
200rpm = 2 x g
• Short acceleration and deceleration
• Easy handling and big buttons
• Shortrun, Softstart and Softstop
- the only one to offer this feature
in its class
• Two acceleration and deceleration
curves
• Motorised lid lock
• Large selection of polypropylene and
aluminium rotors
• Air cooling ensures minimal temperature increase of samples in the
rotor bowl
• Maintenance-free induction drive
motor.
The Sigma 1-14 laboratory centrifuge
operates smoothly and quietly. It is also
very reliable and accurate. ⚛
Wirsam Scientific
Tel:
011-482‑1060 / 021-386‑9020 /
031-709-0199 / 041-365‑2060 / 016-931‑1731
Email: [email protected]
Maximising Sample Preparation
E
limination of particles from samples
prior to HPLC or other chromatographic analysis is essential in order to
maintain integrity of a chromatography
column and to maximise its operating life.
The Sartorius Minisart® syringe filters
consist of a PP housing and membrane
components featuring maximum chemical
compatibility and minimum extractables
to ensure excellent results.
Choose between syringe filters with
hydrophilic, solvent-resistant regenerated cellulose membranes, nylon membranes for rapid small volume filtration
of both aqueous and solvent-based
samples or PTFE membranes with
ultra-high chemical resistance. Minisart
syringe filters allow for fast and easy
UHPLC, HPLC and GC sample preparation with maximum convenience.
These syringe filters are identified
with the pore size and type of membrane on the filter housing.
They also have low adsorption of analytes maximising recovery of product
and broad chemical compatibility with
superior flow rates, and low hold-up
volumes.
All filters are 100% integrity-tested,
bidirectional and are supplied with a
Certificate of Quality. The units can be
fitted on standard syringes.
The Minisart syringe
filters feature:
• Instruments that are handy and
readily available
• Filters that are extremely easy to use
• Proven quality
• An economical way to eliminated
unwanted particles from samples
• Wide array of different types and sizes
• 100 % tested during the manufacture
of these syringe filters. ⚛
National Separations
Contact: Frances Renwick
Tel: 011-553-2300
Email: [email protected]