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BOLT Frequently Asked Questions: Multi-breed Genetic Evaluation powered by BOLT By IGS Genetic Evaluation Team The new genetic evaluation, Multi-breed Genetic Evaluation powered by BOLT, offers groundbreaking advances in the prediction of EPDs for the IGS group. Here are some frequently asked questions and answers to help you better understand the new evaluation. 1. What are the key features of the Multi-breed Genetic Evaluation powered by BOLT? • Faster and more automated system allowing for frequent gene • Improved use of genomic data with Single-step. • Improved methodology for predictions of all traits. • More accurate accuracy. • More flexibility to add additional traits or change methods for future improvements. 2. How is IGS’s single-step approach different from blending for genomic evaluation? The blending approach uses separate steps to calculate genomically enhanced EPDs. This approach requires two steps. The first step is to estimate the effects of DNA markers through a process called “training” or “calibration”. These effects are then used to calculate molecular breeding values (MBVs) on genotyped animals. The MBVs are then combined with traditionally calculated EPDs to enhance the accuracy of 60 | JUNE/JULY 2018 the traditionally calculated EPDs. The blending process is only performed on genotyped animals. Befitting its name, the single-step approach calculates genomically enhanced EPDs in one step — using DNA, pedigree information, and phenotypes simultaneously. As a result, the DNA information not only improves the accuracy of prediction on genotyped animals, but also on the relatives and contemporaries of the genotyped animals. In a sense, all animals are genomically enhanced under the single-step approach. There are also issues inherent in the blending process that are solved with single-step. Similar to the fact that only reporting phenotypes on a selected group of animals in your herd can lead to less informative (and more biased) EPDs with traditional evaluation, problems can exist with blending as it only involves genotyped animals — and genotyped animals tend to be highly selected. However, because single-step includes information from non- genotyped as well as genotyped animals, the issues are corrected. 3. How is the Multi-breed Genetic Evaluation powered by BOLT different than other single- step models used in other genetic evaluations? It is well established that DNA markers vary greatly in their effect on traits — ranging from a large to no impact. To leverage this biological fact in a statistically advantageous manner, the BOLT single-step method only utilizes markers that have a meaningful impact on the traits of interest, while ignoring those that have little to no