Gel Electrophoresis
Uses gel electrophoresis to
separate native or denatured
proteins by the length of the
polypeptide (denaturing
conditions) or by the 3-D
structure of the protein (native /
non-denaturing conditions). The
proteins are then transferred to a
membrane (typically
nitrocellulose or PVDF), where
they are probed (detected) using
antibodies specific to the target
protein.