infectious diseases
New VPD Reference Center PCR Assay to Rapidly Identify Measles Vaccine Strain Now Available
By Kevin Bradley , senior specialist , Infectious Diseases
In public health investigations , rapid detection and characterization of pathogens facilitates timely implementation of appropriate response measures critical to curtailing the spread of disease . Seven to 11 days following receipt of the MMR vaccine , some recipients exhibit a mild form of measles not thought to be contagious . Distinguishing vaccine reactions from wild type virus saves time , resources and focuses public health efforts on true cases potentially associated with outbreaks .
The
APHL / CDC Vaccine Preventable Disease ( VPD ) Reference Centers are implementing a new real-time reverse transcription PCR ( RT-qPCR ) assay ( MeVA ) that is highly specific for the non-circulating measles virus strains of genotype A , the viruses used in the production of the Measles Mumps Rubella ( MMR ) vaccine .
1 This assay will be used along with the more sensitive measles virus ( MeV ) RT-qPCR diagnostic assay to rule out vaccine-related cases from outbreak investigations .
Until now , identifying a vaccine-related virus was a two-step process , first detecting by MeV RT-qPCR and then genotyping by sequencing . Eliminating the second step for known genotype A specimens decreases turnaround time for identifying a vaccine-related virus up to one week , providing the opportunity for a more efficient public health response .
The test will be available to other public health laboratories in the first quarter of 2018 . APHL will send a communication announcing test availability and instructions for ordering the test once it is deployed at all VPD Reference Centers . n
References
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M100 | Performance Standards for Antimicrobial Susceptibility Testing , 28th Edition
M02 | Performance Standards for Antimicrobial Disk Susceptibility Tests , 13th Edition
M07 | Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically , 11th Edition
On Demand Webinar | CLSI 2018 AST Webinar : M100 , M02 , and M07 Updates For package options and to order , visit clsi . org / ast2018 .
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Winter 2018 LAB MATTERS 3 |
infectious diseases
New VPD Reference Center PCR Assay to Rapidly
Identify Measles Vaccine Strain Now Available
By Kevin Bradley, senior specialist, Infectious Diseases
In public health investigations,
rapid detection and characterization
of pathogens facilitates timely
implementation of appropriate response
measures critical to curtailing the
spread of disease. Seven to 11 days
following receipt of the MMR vaccine,
some recipients exhibit a mild form of
measles not thought to be contagious.
Distinguishing vaccine reactions from
wild type virus saves time, resources and
focuses public health efforts on true cases
potentially associated with outbreaks.
The APHL/CDC Vaccine Preventable
Disease (VPD) Reference Centers are
implementing a new real-time reverse
transcription PCR (RT-qPCR) assay
(MeVA) that is highly specific for the
non-circulating measles virus strains
of genotype A, the viruses used in the
production of the Measles Mumps
Rubella (MMR) vaccine. 1 This assay will
be used along with the more sensitive
measles virus (MeV) RT-qPCR diagnostic
assay to rule out vaccine-related
cases from outbreak investigations.
identifying a vaccine-related virus up to
one week, providing the opportunity for
a more efficient public health response.
The test will be available to other public
health laboratories in the first quarter of
2018. APHL will send a communication
announcing test availability and
instructions for ordering the test once it is
deployed at all VPD Reference Centers. n
Until now, identifying a vaccine-related
virus was a two-step process, first
detecting by MeV RT-qPCR and then
genotyping by sequencing. Eliminating
the second step for known genotype A
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