Lab Matters Summer 2020 | Page 24

INDUSTRY MATTERS Critical Considerations for COVID-19 Detection by Arvind Kothandaraman, general manager, Specialty Diagnostics, PerkinElmer Finding the right supplier network for a public heath laboratory’s COVID-19 Real-Time PCR test can be a difficult decision. The challenges are unprecedented, and the solutions may seem obscure. The following factors will help laboratories evaluate their supply options and make a clear choice. Assay performance The viral dynamics of SARS-CoV-2, the virus that causes COVID-19, is not yet fully understood. There is evidence that viral load tends to correlate with the severity of infection. Reports also suggest the possibility of COVID-19 transmission from persons who are pre-symptomatic or asymptomatic. Therefore, a highly sensitive diagnostic assay with the ability to detect low viral load is required to accurately detect the virus and assist in preventing the spread of infection. Automation and assay controls are effective tools to ensure robust results. Availability of reagents and equipment Public health laboratories report that supply interruption has had a significant negative impact, resulting in delayed results and decline in requests received over time. Vendors with a steady supply chain and a transparent view into this should be prioritized. Materials can be PerkinElmer Coronavirus Nucleic Acid Detection Kit Relying on the existing onsite support infrastructure and our 15-year relationship with PerkinElmer, we knew we not only had the solutions to set up our own PCR assay, but also the engineers on speed dial to troubleshoot as needed.” Rachel Lee, PhD directly sourced from manufacturers who meet these criteria or from organizations such as the International Reagent Resource (IRR) that source from manufacturers and supply at no cost to eligible organizations like US public health laboratories. Processing capacity Many laboratories plan to increase capacity by adding platforms, shifts or expanding their workforce. With the flu season fast approaching, there is an increasing need to run a flexible operation to cope with fluctuating demand for testing. Modular automation of viral nucleic acid extraction and real-time PCR preparation can help laboratories adapt to variable sample throughput and reduce exposure of staff to the risk of laboratoryacquired infections. Sample-to-answer solution Testing is a complex, multistep process. Suppliers offering a comprehensive, ready-made solution to implement automated COVID-19 testing can simplify life for laboratory staff. A consolidation to an appropriate vendor can improve procurement efficiency and visibility to availability status of specialized reagents and equipment. The Arkansas Public Health Laboratory has been on the front lines in testing its residents for COVID-19 and has increased its testing goals. To meet its requirements, the state has adopted an automated workflow from PerkinElmer, which Arvind Kothandaraman, general manager, Specialty Diagnostics, PerkinElmer has enabled the laboratory to increase samples processed with less staff. Support When laboratories navigate unchartered territory, quality of support can be the difference between success and failure. A responsive and meticulous support team can help guarantee a better experience inside and outside the lab by assisting in every step along the way from installation to analysis. Laboratories should opt to work with suppliers they trust. Rachel Lee, PhD, manager of the Biochemistry and Genetics Branch at the Texas Department of State Health Services, Laboratory Services Section, believes that having a reliable and trusted supplier was integral in ramping up her laboratory’s testing needs. “We were under immense pressure to rapidly increase capacity to meet the testing demand,” she said. “Relying on the existing onsite support infrastructure and our 15-year relationship with PerkinElmer, we knew we not only had the solutions to set up our own PCR assay, but also the engineers on speed dial to troubleshoot as needed.” • PerkinElmer is a Diamond Level sustaining member of APHL. 22 LAB MATTERS Summer 2020 PublicHealthLabs @APHL APHL.org