APHL 2019 POSTER ABSTRACTS
be accomplished, a series of topical presentations followed by
opportunities for participants to engage in small group activities to
discuss issues and provide feedback on how to address community
needs. The intent of this meeting was to move the discussion of
CECs towards a more proactive and holistic plan to address future
CEC issues more effectively. At the conclusion of the meeting,
participants were able to evaluate learning objectives and capture
other important feedback.
The meeting, Science and Policy of Emerging Contaminants in
Minnesota, built on previously held events, and went beyond just
updating the community on CEC work already occurring, and
explored ways in which that work can lead to policies and actions.
Significant insights gained from this meeting included:
• Minnesota has a diverse community of CEC-interested
stakeholders that strive for collaboration and engagement,
• CEC communication to the public is difficult and groups are
motivated to learn about proven techniques for outreach,
• These contaminants in many ways mirror our lifestyles, so the
CECs we identify and prioritize are going to have to grow and
evolve to reflect ongoing lifestyle changes and risks.
Presenter: Paul Moyer, Minnesota Department of Health, St. Paul,
MN, [email protected]
Optimizing Phosphorus-specific Detection for the Analysis
of Glyphosate and Other Organophosphorus Pesticides
Using HPLC-ICP-QqQ-MS
K. Aviado, C. Dingman, J. Schneider, J. Chithalen and C. Bean, New
Hampshire Public Health Laboratories
Organophosphorus pesticides include insecticides and herbicides
employed widely for agricultural use. Many of these compounds
have demonstrated a range of health impacts. Exposures can
occur through contact with contaminated water and agricultural
products, and there is interest in developing analytical methods that
achieve rapid, sensitive, and robust simultaneous measurement
of organophosphorus pesticides and their breakdown products in
a variety of environmental and clinical matrices. We investigate
the application of inductively coupled plasma triple quadrupole
mass spectrometry (ICP-QqQ-MS) to quantitatively measure the
phosphorus heteroatoms shared by these compounds as an
alternative to traditional liquid chromatography-tandem mass
spectrometry (LC-MS/MS) methods that may experience limitations
related to sample derivatization and poor analyte ionization.
Although phosphorus is considered challenging to measure in
conventional argon plasma ICP-MS due to its difficult ionization and
intense mass spectral interferences on the phosphorus detection
mass (m/z = 31), the collision/reaction capabilities of the triple
quadrupole system improve analyte selectivity and sensitivity. We
performed experimental optimizations of the ICP-QqQ-MS collision/
reaction cell using helium, oxygen (O2), argon, and hydrogen gas,
and found that the highest signal-to-noise was obtained by using
O2 as the cell gas and selectively detecting reaction product
ion 31P16O+ at m/z = 47. By coupling high performance liquid
chromatography (HPLC) to the ICP-QqQ-MS system, we investigated
a simple and rapid method for separation and analysis of
glyphosate and its metabolite aminomethyl phosphonic acid (AMPA),
as well as malathion dicarboxylic acid (MDA; the primary metabolite
of malathion) using ion-pairing liquid chromatography.
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Presenter: Kimberly Aviado, New Hampshire Public Health
Laboratories, Concord, NH, [email protected]
Optimization of Testing Methodologies for the Analysis
of Organic Pollutants in Clinical Specimens for a
Biomonitoring Surveillance Study
C. Dingman, K. Aviado, J. Schneider, J. Chithalen and C. Bean, New
Hampshire Public Health Laboratories
The Tracking and Assessment of Chemical Exposures (TrACE) Study
is BiomonitoringNH’s statewide initiative that will provide baseline
data to assess long term and acute environmental chemical
exposures to NH’s residents. The method optimization process
used to analyze the low levels of chemical toxins found in human
biological specimens that are required for analytical quantitation is
lengthy and constantly evolving, but essential for producing robust
exposure data. The analyte panels for the TrACE Study were chosen
based on exposures expected to be found in NH’s environment and
include panels for three sets of metals and three groups of organic
pollutants. Focusing on the organic pollutant analytes, we have a
panel to monitor exposure of 14 short and long-chain perfluoroalkyl
substances (PFAS) from industrial contamination, cotinine from
tobacco, and 10 pesticide metabolites from agriculture. All
three panels undergo various steps of sample cleanup prior to
analysis via high performance liquid chromatography-tandem
mass spectrometry (HPLC-MS/MS). In this study, we describe the
optimization of three diverse sample preparation techniques for low-
level analysis of our target analytes including automation, sensitivity,
runtime, and recovery. The sample preparation techniques for
each of the panels are vastly different but all three have the same
objective: a clean analytical extract void of interferences from matrix
effects and a high, consistent recovery. The exposure data collected
from the TrACE Study is valuable for the participants who receive
their individual results and follow-up options. Likewise, the general
population also benefits from the aggregate data that will be used
by our state public health epidemiologists and The New Hampshire
State Legislature to better identify new exposure events, inform
policy recommendations, prioritize limited public health funds, and
produce educational materials for New Hampshire’s citizens.
Presenter: Carleen V. Dingman, New Hampshire Public Health
Laboratories, Concord, NH, Email: [email protected]
Serum Concentration of Polychlorinated Biphenyls (PCBs)
from New Jersey State-Wide Biomonitoring Study: 2015–2018
S. Du, N. Patterson, C. Chu, C.D. Riker, C.H. Yu and Z. Fan, New
Jersey Department of Health
Polychlorinated biphenyls (PCBs) are persistent pollutants and
detectable amounts are constantly found in blood of most
populations that have been examined. As part of efforts of New
Jersey Department of Health State Biomonitoring program, forty
ortho-substituted Polychlorinated biphenyls (PCBs) are measured in
serum samples collected from New Jersey residence using high-
resolution gas chromatography/isotope-dilution high-resolution
mass spectrometry (HRGC/ID-HRMS). The objectives of this study
are to characterize the concentration level and pattern of PCBs
in New Jersey residence by age, gender and ethnicity and identify
major exposure factors. The lipid adjusted median concentration
of the sum of 40 PCB congeners (ΣPCBs) (N=600) was 168 ng/g
Summer 2019 LAB MATTERS
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