APHL 2018 Annual Meeting Poster Abstracts
Rapid Concentration of Biological Particles from
Environmental Samples
A. Lewis, M. Hornback and A. Page, InnovaPrep, Drexel, MO
InnovaPrep has developed a suite of systems for concentration
of bacteria and other biological particles from liquid samples.
Volumes of water from a few milliliters to tens of liters of water
are processed through flat or hollow fiber membrane filters to
capture any biological particles that are present. The biological
particles are then efficiently recovered from the membrane
surface with a tangential flush using carbonated “wet foam.” The
wet foam is expanded up to six times the o riginal liquid volume
and becomes highly viscous, allowing it to act at the membrane
surface and recover the particles into volumes significantly
smaller than can be attained with traditional liquid elutions. The
process is scalable, efficient and typically results in concentration
factors of approximately 1000X per concentration stage. The CP
Select, launched in September 2017, is the second generation of
InnovaPrep’s Concentrating Pipette (CP) instrument. The CP Select
uses Concentrating Pipette Tips (CPT) to aspirate and concentrate
bacteria from a large input sample and then dispense a final
concentrated sample. CPTs are disposed of after each sample,
enabling the CP to be utilized for concentration of pathogens and
indicator organisms from a variety of aqueous samples without
cross contamination. Two types of flat membrane filter CPTs and
four types of hollow fiber membrane filter CPTs, allow the system to
be used for a variety of sample types and a variety of organisms,
including viruses, bacteria and parasites. Concentration of 100 mL
water sample and 1 L water sample, experimentally spiked with
100 CFU Escherichia coli were performed using InnovaPrep’s CP
Select liquid concentrator using 0.2 µm hollow fiber Concentrating
Pipette Tips (hf-CPTs) and 0.075% Tween 20/PBS elution fluid.
Concentration of 100 ml spiked PBS resulted in an average
recovery efficiency of 78.8% in the first elution, with an average
elution volume of 337 µL and an average processing time taking
0.8 minutes. Recovery efficiencies for 1 L spiked PBS were similar
to data from 100 mL runs, with 75.3% recovery in an average of
362 µL elution volume. Overall recovery increased to 85.19% when
combined with a second elution. Overall time to concentrate 1 L of
spiked fluid took 7.22 minutes.
Presenter: Ariel Lewis, InnovaPrep, Drexel, MO, Phone:
816.619.3375, x104, Email: [email protected]
of preparedness measures for healthcare partner response to
public health emergencies, such as chemical threats. Robust Level
3 programs, developed by state member laboratories, include
healthcare partner training, exercises and outreach. Whether a state,
territorial or metropolitan area, each LRN-C laboratory is unique with
regard to demographics, size and hospital system. To aid Level 3
Coordinators to fill any gaps in their programs, a resource guidance
document was proposed by CT-CWG members. This document
would include practical information that could be used to develop
a robust Level 3 program with consideration for each jurisdiction’s
specific needs. APHLs Environmental Health Committee (EHC)
and eleven volunteers from the CT-CWG provided subject matter
expertise for development of a universal guidance document for
the Level 3 Coordinators. Meetings hosted by APHL members were
held utilizing an APHL SharePoint site for efficient coordination of
conference calls and email. The group established experience based
recommendations that would be most helpful for Level 3 activities.
The group also solicited ideas from the entire APHL CT-CWG. The
workgroup met frequently to discuss progress and suggest edits for
improvements to the Level 3 Resource Handbook. Since the first
version release, minor revisions were made to the document in
March 2015. December 2016, the EHC proposed a refresh of the
Level 3 Resource Handbook. Short term goals included addition of an
acronym list, repairs to URLs and Emergency Response Laboratory
Network (ERLN) information. Long term goals were identified for the
training section of the guidebook that would include enhancement
of training program practices and an exercise experience library
as portrayed by the different member states. Short term revisions
were completed in September 2017 by a smaller group of CT-CWG
members using similar communication paths developed during the
making of initial version. Our poster will announce and share the
remake of the (LRN-C) Level 3 Resource Handbook.
Presenter: Teresa Miller, Michigan Department of Health and
Human Services, Lansing, MI, Phone: 517.241.0925, Email:
[email protected]
Detection and Characterization of Salmonella
in Environmental Samples
(complete abstract in Food Safety, p. 57)
Triple Quadrupole ICP-MS for Biomonitoring Applications
Laboratory Response Network — Chemical (LRN-C) Level 3
Resource Handbook K. Aviado, C. Dingman, J. Schneider and C. Bean, New Hampshire
Public Health Laboratories, Concord, NH
S. Yerabati 1 , T. Miller 2 , R.E. Nickla 3 ; 1 California Department of Public
Health, Richmond, CA, 2 Michigan Department of Health and Human
Services, Lansing, MI, 3 Oregon State Public Health Laboratory,
Hillsboro, OR Triple quadrupole inductively coupled plasma mass spectrometry
(ICP-QqQ-MS) offers many advantages over traditional single
quadrupole (ICP-Q-MS), including enhanced analyte selectivity,
sensitivity and matrix tolerance, making it a useful tool for
performing highly sensitive trace elemental measurements in a
variety of clinical matrices for biomonitoring public health studies.
The Biomonitoring Program at the New Hampshire Public Health
Laboratories (NH PHL) employs triple quadrupole ICP-MS to assess
exposures to trace metals in communities around the state. The
NH PHL has developed several analytical methods on a triple
quadrupole (ICP-MS) to conduct two ongoing public health studies:
a targeted investigation that assesses exposure to arsenic and
uranium from private well water in high-risk communities around
the state and a surveillance study that monitors for toxic metals
The APHL Chemical Threat Collaborative Workgroup (CT-CWG)
developed the Laboratory Response Network-Chemical (LRN-C) Level
3 Resource Handbook. The guide was designed to be used by LRN-C
member partners and coordinators who provide Level 3 outreach to
healthcare facilities, first responders and any other agency expected
to respond to a chemical release resulting in human exposure. The
first version was released in November 2014 and was placed on
the LRN-C Toolkit APHL SharePoint site. Specific funded benchmark
Level 3 capabilities, as defined by the Centers for Disease Control
and P revention (CDC), should be sustained, including the provision
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LAB MATTERS Summer 2018
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