APHL 2022 POSTER ABSTRACTS
Liquid Chromatography ( LC ) conditions and Mass Spectrometry ( MS ) parameters as well as Supported Liquid Extraction ( SLE ) procedure to enhance overall method sensitivity / selectivity . By using new mobile phase compositions and switching to different ionization source , we achieved much higher signal to noise ratio ( S / N ), which resulted in LOQ ( Limit of Quantitation ) improvement from 0.02ng / mL to 0.005 ng / mL . In addition , we further refined the sample preparation steps that resulted in recoveries greater than 95 % for both analytes in complex human serum matrix . This optimized method was validated to achieve excellent accuracy ( 90-105 %) and precision (< 5 %). This validated method will be used to support the NJ Health and Nutrition Examination Survey ( NJHANES ), ongoing NJ population surveillance study for the NJ State Biomonitoring Program . The method optimization process and validation results will be discussed in this poster .
Presenter : Carrie Xu , New Jersey Public Health & Environmental Laboratories , carrie . xu @ doh . nj . gov
The Analysis of Hydroxy Polycyclic Aromatic Hydrocarbons by Isotope Dilution Online Solid Phase Extraction Followed by High Performance Liquid Chromatography Tandem Mass Spectrometry
E Cook , CH Yu , AM Marcel , S O ’ Leary , Z Fan and L Zhong , New Jersey Public Health & Environmental Laboratories
The New Jersey public health laboratory is a recipient of the national biomonitoring grant for the second year . The goal of the grant is to perform biomonitoring in New Jersey ( NJ ). Surveying concentrations of toxic elements and chemical compounds in NJ is important to understand human exposure . Polycyclic aromatic hydrocarbons ( PAHs ) are produced from incomplete combustion processes . These PAHs are widespread and many are suspected carcinogens with no established threshold levels for carcinogenicity . Biomonitoring of PAHs is relevant for environmental public health because of the widespread exposure of PAHs to humans . Some PAHs are metabolized in the human body and excreted in urine . Eight hydroxy polycyclic aromatic hydrocarbons in human urine can be quantitated by isotope dilution , online solid phase extraction ( SPE ), followed by high performance liquid chromatography tandem mass spectrometry HPLC-MS / MS ). The concentration of eight PAHs ( 1-hydroxynaphthalene , 2-hydroxynaphthalene , 2-hydroxyfluorene , 3-hydroxyfluorene , 1-hydroxyphenanthrene , 2- & 3-hydroxyphenanthrene , 1-hydroxypyrene ) in human urine can be determined for 500 subjects as part of the New Jersey biomonitoring program . The reference CDC method4 is adapted for our protocol . 100 µ l of human urine is combined with 50 µ l internal standard , 50 µ l enzyme buffer , 20 µ l ascorbic acid solution and 200 µ l MeOH . This mixture is shaken for 10 minutes at a frequency of 23 / s . After incubating the mixture for 18 hours at 37 ° C , 200 µ l of MeOH is added and centrifuged for 15 min at 7500 rpm to remove the enzyme . 200 µ l of the supernatant is added to a 500 µ l autosampler vial containing 350 µ l water . This solution is shaken for 10 minutes at a frequency of 23 / s , before injection . Sample SPE , separation and analysis if performed on a ABSciex Qtrap 6500 and spark Holland SPE / HPLC system . SPE is performed on 450 µ l of specimen . The analytes are separated using by an Agilent Zorbax Eclipse PAH column analyzed in the mass spectrometer . The chromatography of the samples showed good separation of all eight analyte by the gradient program employed . The calibration range for 1 & 2-hydroxynaphthalene is between 200 – 0.0488 ng / ml and all other analytes are between 25.0 – 0.00610 ng / ml . The r2 values for all analytes are 0.99 and greater . The method blanks are less than the lowest calibration for each analyte . The limit of detection , accuracy and precision is currently being determined .
Presenter : Linbin Zhong , New Jersey Public Health & Environmental Laboratories , Linbin . Zhong @ doh . nj . gov
South Carolina Opioid Bio-Surveillance Outreach Program M Tredway , South Carolina Public Health Laboratory
South Carolina ( SC ) was awarded grant funding from the Centers for Disease Control and Prevention ( CDC ) to gather data on suspected opioid overdoses . With funding from the Overdose Data to Action Cooperative Agreement ( OD2A ), the SC Public Health Laboratory ( PHL ) is working with hospitals to test de-identified urine specimens from suspected opioid overdose patients . The SC PHL tests the specimens for twenty-one fentanyl analogs and reports the data to participating hospitals and stakeholders . The data is helping our state in detecting and addressing opioid misuse in collaboration with our Injury and Substance Abuse Prevention Division and partners statewide . An aggressive recruitment campaign was developed to partner with local hospitals to assist SC PHL by providing residual urine specimens for analysis .
Objectives : To enter into a partnership with hospitals and freestanding Emergency Departments under a formal Memorandum of Agreement ( MOA ). Train hospitals on specimen submission , provide logistical support and report data . These partnerships allowed for data collection that will provide information on opioid usage and trends in SC .
Methods : Initially , SC PHL staff attempted to get hospital participation by working with hospital laboratory directors , with limited success . After redesigning the approach and targeting hospital executive staff and key stakeholders , we were able to achieve engagement and MOA partnerships at a rate above 90 %.
Results / Conclusions : The SC PHL has successfully recruited 37 out of 63 hospitals and two free standing Emergency Departments . The recruitment has resulted in the SC PHL receiving over 5,300 specimens for analysis , thus providing the state with valuable data in addressing the opioid epidemic . Our recruitment campaign has provided data that is used by hospitals , communities , law enforcement , public health officials and other state agencies to fully understand the true impact of opioid use in SC . Our ongoing engagement efforts will continue to build relationships that will help retain partnered hospitals and expand the testing capabilities of the SC PHL .
Presenter : James LaPalme , South Carolina Public Health Laboratory , Email : lapalmjh @ dhec . sc . gov
Detection of Mitragynine and 7-Hydroxymitragynine in Keratinized Specimens
A Thompson and J Jones , United States Drug Testing Laboratories , Inc .
Environmental Health