ABSTRACTS OF POSTER COMPETITION WINNERS
1st Place Winner
Title:
A New Class of Matrix Metalloproteinase
Inhibitor Preserves Dentin Bond
Presenter:
Kazuma Kitagaito
University of Pennsylvania
School of Dental Medicine
240 South 40th Street, #7461
Philadelphia, PA 19104
(650) 336-0077
[email protected]
Authors:
Kazuma Kitagaito1, Nuray Tuloglu2, David Persky3,
Haritha Ghanta1, Markus B Blatz1, Abdulhafez Selim3,
Marina D’Angelo3, Fusun Ozer1
1. University of Pennsylvania, Philadelphia, USA
2. Osmangazi University, Eskisehir, TURKEY
3. Philadelphia, College of Osteopathic Medicine,
Philadelphia, USA
Objective:
A novel class of therapeutic, the Extracellular Matrix Protection
Factor (ECPF), is a selective MMP inhibitor targeting specific
family members. This study investigated the influence of
MMP-8 selective inhibitor pretreatment on preserving
dentinal bond strength after one year in-vitro aging.
Methods:
36 human molars, preserved in -80°C, were prepared
by creating flat occlusal dentin surfaces for box cavity
preparations measuring 5x3x1.5mm. All teeth were
randomly divided into three groups (n=12) according to
their pretreatment procedures: Control (NT), chlorhexidine
(CHX), and ECPF. Prior to pretreatment, all cavities underwent
acid etching with 37% H3PO4 (30s). For CHX and ECPF
groups, pretreatment of 2% CHX (60s) or 5μg ECPF (60s)
followed etching. All teeth were restored using bonding
agent (Prelude-one, Danville Materials) and composite-resin
built up.
Groups were separated into two sets randomly: immediate
(IM) and thermo-mechanical aging (TMA). IM set underwent
microtensile bond strength (μTBS) testing after 24 hours
water storage. For TMA set, the teeth were exposed to
thermocycling regimen of 20,000 cycles between 5oC
and 55oC, and placed in chewing simulator (240,000
cycles; 50N) to simulate one year of oral conditions. The
restorations were vertically sectioned to obtain 1.0 mm2
cross-sectional composite-dentin beams. μTBSs were
measured. Data were analyzed by two-way ANOVA and
Tukey’s test.
Results:
μTBS [MPa]
Mean±SD
Groups
NT
CHX
ECPF
IM
20.4±4.1B,a
20.3±3.3B,a 39.6±8.1A,a
TMA
21.4±5.6B,a
24.0±7.5B,a
28.9±4.6A,b
Different superscript uppercase letters (in row) and different superscript lowercase
letters (in column) indicate statistically significant differences (p<0.05).
Bond strengths of NT and CHX groups were similar for
both IM and TMA sets. However, strengths for both
immediate and aged ECPF groups were statistically
different against all NT and CHX groups.
Conclusion:
The application of ECPF pretreatment increased dentinal
bond strength after one year in-vitro
aging, which yields clinically meaningful differences.
However, further investigations are indicated to improve
dosage and delivery methods.
Funding/Conflict of Interest:
Funding: University of Pennsylvania School of Dental
Medicine Schoenleber Pilot Grant
J U LY / A U G 2 0 1 6 | P E N N S Y LVA N I A D E N TA L J O U R N A L
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