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Cardiovascular disease in cerebral palsy these findings in the context of the general population. A longitudinal study completed in older adults (mean age 60 ± 9 years) found that cfPWV increased from 9.6 to 10.4 m/s in a 4–6-year time-period (20). Com- parably, we found a modest increase in cfPWV from 6.2 to 6.9 m/s in a similar time period (i.e. 4 years), but in a much younger cohort of individuals with CP (mean age 31.2 ± 15.0 years at baseline). In addition, there is well-established literature demonstrating that advancing age is the strongest predictor of increases in cfPWV (18, 21), and we previously found age to be significantly associated with cfPWV in a cross- sectional analysis of 42 adults with CP (8). However, our current multiple regression analysis did not reveal age to be associated with rate of change in cfPWV in the present study, which, in addition to younger age at baseline, might be explained by a combination of the small magnitude of observed changes in cfPWV and the small sample size. Similarly, cIMT increases with age due to changes to the structural components of the arterial wall, particularly thickening of both the intima and media layers; in the general population, cIMT increases by approximately 3-fold between the ages of 20 and 90 years (22). The 1 st Screening for Heart Attack Prevention and Education (SHAPE) Program identified an individual to be at high risk for subclinical atherosclerosis when a cIMT ≥ 1 mm is present (23). Considering the information in the SHAPE guideline, 2 individuals in the present study were at high risk for subclinical atherosclerosis from cIMT alone. A study in a cohort of 70-year-old Swedish adults found that cIMT significantly increased from 0.88 to 0.95 mm over a 5-year period (24). The present study found a larger relative increase in cIMT compared with the Swedish cohort. Also, age was a strong significant predictor of cIMT change in our study, which is consistent with what is known in the general population and further supports our findings from cross-sectional work (8). In addition to age, another important variable to consider when examining CVD risk is hormonal development. Specifically, literature has shown that estrogen might have a cardioprotective effect on the cardiovascular system (25); however, this topic remains controversial (26). While this was the first longitudinal study that examined cardiovascular health in individuals with CP and demonstrated change over time, we recommend investigating the effect of age and sex, in particular metabolic and hormonal effects on cardiovascular health, in future studies. Few studies have examined longitudinal changes in brachial artery FMD from adolescence to adulthood. A study in children and adolescents with type 1 diabetes mellitus observed a significant decrease in FMD in ap- proximately a 3-year follow-up period (27); whereas no 529 significant change in FMD was observed after a 5-year follow-up study in older adults (24). The latter study observed a significant inverse relationship between change in FMD and change in LDL-cholesterol (24), whereas the former study observed an inverse relation- ship between glycaemic control and FMD (27). It is plausible that metabolic markers could be associated with change in FMD in individuals with CP; however, we did not measure these in the current study. At the very least, we know that age and gross motor function were not significantly associated with change in FMD in the present cohort. Given the recent work by Peter- son et al. (28) and Ryan et al. (29) pointing towards adolescents and adults with CP having an elevated risk of cardiometabolic disease, it is important for future work to include cardiometabolic markers to advance our understanding of the regulation of endothelial fun- ction in this population. Indeed, we did observe a (non- significant) increase in waist circumference; however, our modest sample size limited our ability to include waist circumference as an independent predictor in li- near regression analysis. Finally, a significant decrease (1.1E–3 mmHg –1 ) in carotid artery distensibility bet- ween baseline and follow-up assessment was observed in our cohort. In a large cohort of men and women free of CVD (45–84 years old), carotid artery distensibility decreased by 0.41E–3 mmHg –1 over a mean period of 9.4 years, and SBP was associated with accelerated stiffening (30). A larger decrease in distensibility that was not associated with age or gross motor function was observed. Taken together, our findings provide initial support for the argument that individuals with CP experience accelerated ageing for disease progres- sion, specifically non-traditional CVD risk factors, in comparison with the general population. In light of recent research that identified young adults with CP at increased risk of CVD compared with age-matched controls from the general population (31), early de- tection of the development of CVD, particularly by assessing non-traditional risk factors for CVD, can lead to more effective strategies for the prevention of CVD in this population. Study limitations There are important limitations to address in this cohort study. The main limitation was the small sample size. While the sample size of 28 individuals did allow us to examine longitudinal changes in CVD risk factors in the total group, we were unable to determine whether other factors (e.g. waist circumference and sex hor- mones) were associated with the observed changes in CVD risk variables. Secondly, the large age range would have been best examined in age groups with a J Rehabil Med 51, 2019