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440 B. J. H. van Lith et al. baseline was based on tests with a known perturbation direction. As a consequence, the perturbation intensities might have been too high for the participants following perturbations in unknown directions. This may also (partly) explain the much lower number of participants able to sustain perturbations with unknown compared with known directions. Another reason might be that reactive steps following unexpected perturbations are relatively strongly influenced by delayed postural re- sponses that occur in patients with HSP (4, 26), because patients cannot compensate by an anticipatory “central set” if the direction is unknown. Nevertheless, the improved balance capacity following perturbations in known directions suggests that hip adductor spasticity indeed impairs the quality of lateral stepping responses and, thus, is a relevant treatment target from a frontal- plane balance perspective. The fact that the beneficial effects on muscle tone, gait width, comfortable gait velocity, lateral balance persisted until 16 weeks after treatment seems to chal- lenge the common opinion that the biological effects of BTX-A have worn off after this time interval. It may, therefore, be that the stretching component of the treatment protocol was responsible for the observed long-term effects. Study limitations and future perspectives A limitation of the present exploratory study in pa- tients with HSP is the relatively small sample size and the lack of a control condition, while the rather stringent inclusion and exclusion criteria limit the generalizability of our findings. Nevertheless, this study provides indications for the beneficial effects of bilateral BTX-A injections in the adductor longus, adductor magnus and gracilis muscles and subsequent stretching of these muscles on gait width, comfortable gait speed, and reactive lateral stepping in known perturbation directions, whereas maximal gait speed, gait endurance, and clinical balance scores appear to be less responsive in these patients. Future research should preferably be multi-centred to increase the number of participants and use a randomized controlled design. Gait width and gait speed would be valuable and responsive outcome measures in such trials. The instrumented balance assessments used in the present study are, however, less suitable for multi-centre stu- dies as they require further development of clinically affordable systems. Clinically applicable assessments to validly test the quality of (lateral) reactive stepping responses are therefore urgently needed. As HSP is a chronic and progressive condition, it would also be relevant to conduct longitudinal, comparative cohort studies to investigate whether repetitive cycles of BTX- www.medicaljournals.se/jrm A treatment of spastic hip adductors improve lifetime functional ambulation prognosis in these patients. ACKNOWLEDGEMENT This investigation was supported by an unrestricted grant from Merz Pharmaceuticals. The authors have no conflicts of interest to declare. REFERENCES 1. Hesse S, Werner C, Bardeleben A, Brandl-Hesse B. Ma- nagement of upper and lower limb spasticity in neuro- rehabilitation. Acta Neurochir Suppl 2002; 79: 117–122. 2. de Niet M, de Bot ST, van de Warrenburg BP, Weerdesteyn V, Geurts AC. Functional effects of botulinum toxin type-A treatment and subsequent stretching of spastic calf mus- cles: a study in patients with hereditary spastic paraplegia. J Rehabil Med 2015; 47: 147–153. 3. Gracies JM, Singer BJ, Dunne JW. 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