J. Eur. Opt. Society-Rapid Publ. 22, 34( 2026) 347
Sensor biosensing resolution Concentration of biomolecules
¼
Sensor response Sensor measurement stability: ð1Þ
Furthermore, the observed binding kinetics difference between A1 and C3 sensor spots may cause by spatial non-uniformity of MPXV antibody molecules within the flow cell( 1.5 1.5 cm 2). Such variations could be minimized by using a syringe pump to maintain a continuous flow during detection. In addition, different concentrations of MPXV neutralizing antibodies ranged from 0 ng / lL( PBS buffer), 1.56 ng / lL, 3.125 ng / lL, 6.25 ng / lL to 12.5 ng / lL were sequentially injected onto the MPXV protein functionalized surface. Each antibodies sample was incubated for 45 min within the detection chamber to facilitate MPXV protein – antibody binding interactions. After subtracting the PBS baseline, the normalized sensor responses were plotted in Fig. A1( Appendix). The results demonstrate a linear relationship between the sensor signals and MPXV neutralizing antibodies concentrations. Slope of the sensor response curve also provides the detection sensitivity of the biosensor. It is determined to be 8.45 ng / lL / Hue units.
Acknowledgments
Chi Lok Wong would like to give his sincere thanks to Yeshua for his guidance and supports in all experiments and ideas.
Funding
This research was funded by National Science and Technology Council( NSTC), Taiwan ROC grant number NSTC 112 – 2221- E-182-054- and NSTC 113 – 2221-E-182-010-.
Conflicts of interest The authors have nothing to disclose.
Data availability statement
Data underlying the results presented in this paper can be obtained from the author upon reasonable request.
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