JADE Student Edition 2020 October 2020 | Page 14

With regards to effective cleaning , experiment 1 and preliminary had the worst cleaning result as they were only washed once . This suggests that even at 60 degrees , some stains remained . Both the non-biological and biological laundry capsules can remove bloodstains , but biological capsules did a better job as the intensity across the preliminary and experimental conditions were less for these capsules . The use of pixels in this study helped with providing a quantitative analysis of chemiluminescent . Initial analysis of interpreting luminol emission involved providing qualitative statements using a verbal scale of 0 to 5 where 0 is no chemiluminescence and 5 being intense . However , this was abandoned in favour of software investigations . Studies by Tobe , Watson and Daéid ( 2007 ) and Cassidy et al ( 2017 ), used statistical testing or computer software to analyse their results . Since DNA profile was not gathered in this project , statistical analyses were not undertaken , and instead the software package COREL Photo Paint was used . It provided useful information on the number of pixels available in the photographs in the form of exposure histograms . Better analysis would have involved cropping the pictures to show the reaction area but this wasn ’ t fruitful due to low or no visibility of the samples in the controls . Further studies on blood detection in crime scenes should consider the use of visible reflectance hyperspectral imaging . This can reduce the use of luminol to detect blood as large quantities of luminol can be expensive and time consuming . For example , Edelman , van Leeuwen and Aalders ( 2015 ) used 4 different visible reflectance hyperspectral methods , in the range 400- 720nm to enhance and contrast bloodstains on 12 different fabrics . This method proved to be more successful than white light photography as blood was detected on all fabrics and are also convenient for crime scenes as the camera system is portable and wireless . Hyperspectral imaging can also be used to provide age estimation of blood stains ( Edelman , van Leeuwen and Aalders , 2015 ) thus providing vital clues about the date of crime which luminol , currently , is unable to do so . Along with hyperspectral methods , infrared ( IR ) spectroscopy can also successfully be used to provide contrast between latent bloodstains and the background ( Lin et al ., 2007 ). Future Research
Conclusion
The aims of this project have been met successfully . The project showed that luminol is indeed a sensitive solution and can detected latent bloodstains on swatches that have been washed up to three times and using two different laundry detergents . The results of this project also indicated that elimination of blood in fabrics proved to be a difficult task as chemiluminescence was detected even after three washes . This suggests that the fibres used in the swatches are strong as it proved difficult to get rid of the blood even after multiple washes . The use of pixels gathered from photographic evidence is a novel way of quantifying chemiluminescence intensity and helps with understanding the sensitivity of luminol . Further studies should ponder upon the integration of pixels in quantifying chemiluminescence to improve up the reliability of this technique . References Adair , T . W . and Shaw , R . L ., 2005 . Enhancement of bloodstains on washed clothing using luminol and LCV reagents . IABPA News , [ online ] 21 ( 4 ), pp . 4-10 . Available at : < https :// www . iabpa . org / uploads / files / iabpa % 20publications / December % 202005 % 20News . pdf > [ Accessed 02 January 2020 ]. Bell , S ., 2014 . Forensic chemistry . 2nd ed . Harlow : Pearson .
that dirt from the machines could have transmitted to the swatches . The best chemiluminescence detection was found in the polyester material .

With regards to effective cleaning , experiment 1 and preliminary had the worst cleaning result as they were only washed once . This suggests that even at 60 degrees , some stains remained . Both the non-biological and biological laundry capsules can remove bloodstains , but biological capsules did a better job as the intensity across the preliminary and experimental conditions were less for these capsules . The use of pixels in this study helped with providing a quantitative analysis of chemiluminescent . Initial analysis of interpreting luminol emission involved providing qualitative statements using a verbal scale of 0 to 5 where 0 is no chemiluminescence and 5 being intense . However , this was abandoned in favour of software investigations . Studies by Tobe , Watson and Daéid ( 2007 ) and Cassidy et al ( 2017 ), used statistical testing or computer software to analyse their results . Since DNA profile was not gathered in this project , statistical analyses were not undertaken , and instead the software package COREL Photo Paint was used . It provided useful information on the number of pixels available in the photographs in the form of exposure histograms . Better analysis would have involved cropping the pictures to show the reaction area but this wasn ’ t fruitful due to low or no visibility of the samples in the controls . Future Research

Conclusion

The aims of this project have been met successfully . The project showed that luminol is indeed a sensitive solution and can detected latent bloodstains on swatches that have been washed up to three times and using two different laundry detergents . The results of this project also indicated that elimination of blood in fabrics proved to be a difficult task as chemiluminescence was detected even after three washes . This suggests that the fibres used in the swatches are strong as it proved difficult to get rid of the blood even after multiple washes . The use of pixels gathered from photographic evidence is a novel way of quantifying chemiluminescence intensity and helps with understanding the sensitivity of luminol . Further studies should ponder upon the integration of pixels in quantifying chemiluminescence to improve up the reliability of this technique . References Adair , T . W . and Shaw , R . L ., 2005 . Enhancement of bloodstains on washed clothing using luminol and LCV reagents . IABPA News , [ online ] 21 ( 4 ), pp . 4-10 . Available at : < https :// www . iabpa . org / uploads / files / iabpa % 20publications / December % 202005 % 20News . pdf > [ Accessed 02 January 2020 ]. Bell , S ., 2014 . Forensic chemistry . 2nd ed . Harlow : Pearson .

Brenzini , V . and Pathak , R ., 2018 . A comparison study of the detection of bloodstains on painted and cleaned surfaces with luminol . Forensic science international , [ e-journal ] 289 , pp . 75-82 . https :// doi . org / 10.1016 / j . forsciint . 2018.04.043 14