JADE Student Edition 2019 JADE JSLUG 2019 | Page 36

hypoxic pulmonary vascular response in HAPE- susceptible individuals (Bärtsch et al., 1991; Bärtsch et al., 2005; Bärtsch and Gibbs, 2007). Mechanisms for the hypoxic constriction of arterioles resulting in edema include irregular vasoconstriction with regional over-perfusion and reduced fluid clearance from the alveolar space. decreased incidence of HAPE from 74% to 33% providing further evidence for the inhibition of ENaC in HAPE pathology (Vivona et al., 2001; Sartori et al., 2002; Sartori et al., 2004; Bärtsch and Gibbs, 2007). Hypoxic pulmonary vasoconstriction (HPV) is the homeostatic mechanism responsible for vasoconstriction of intrapulmonary arteries in response to alveolar hypoxia; at high altitude the hypoxia is environmental, thus HPV is diffuse. Diffuse HPV results in increased PAP and initiates pulmonary hypertension (PH) contributing to HAPE development. ROS release is dynamically changed by the mitochondria in the pulmonary artery smooth muscle cells (PASMC) in response to alveolar hypoxia which results in vasoconstriction of the intrapulmonary arteries. Non-uniform pulmonary vasoconstriction results in increased intravascular pressure, and thus, perfusion of fluid across the intrapulmonary artery membranes leading to abnormal accumulation of fluid in the lungs – pulmonary edema. Reduced bioavailability of the messenger molecules, NO and cGMP, is thought to contribute to increased HPV. Increased expression of endothelial nitric oxide synthase (NOS) – responsible for producing NO – has been shown to inhibit hypoxia-induced dysfunction of the endothelium (Bärtsch et al., 2005; Bärtsch and Gibbs, 2007). The classical understanding of acclimatisation describes the restoration of oxygen delivery back to sea-level values. Oxygen delivery is considered to be the product of cardiac output – the product of heart rate and stroke volume – and arterial oxygen content –the sum of oxygen bound haemoglobin (Hb) and plasma dissolved Hb. As previously stated, ascent to altitude without acclimatisation results in reduction of arterial PO2 and SO2, and thus a reduction in oxygen delivery. Physiological changes to counteract this reduction according to the classical explanation include; increasing cardiac output, restorating arterial SO2, and increasing Hb concentration (Martin and Windsor, 2008). The classical explanation of acclimatisation is dependent on the improvement of oxygen delivery to the tissues, however, there are other mechanisms capable of restoring the physiological oxygen balance. These mechanisms include reducing cellular oxygen consumption and improving metabolic efficiency for the generation of energy. Active transport of charged sodium atoms (Na+) across the alveolar epithelium is necessary to ensure fluid-free lungs. Vivona et al (2001) demonstrated hypoxia-inhibited activity and expression of the alveolar epithelial cell Na+ transporters in rats. The effect was particularly pronounced in the apical membrane epithelial Na+ channel (ENaC) and the basolateral membrane Na+/K+-ATPase resulting in a reduction of Na+ transport and fluid clearance from the alveoli, via the alveolar epithelial membrane. Sartori et al (2002) found that prophylactic stimulation of the ENaC transport mechanism increased Na+ transport across the alveolar epithelium and alveolar fluid clearance resulting in Rapid ascent to altitude is the primary risk factor in the development of altitude sickness therefore reduced ascent rate is considered to be the most effective method for altitude sickness prevention. Acclimatisation involves a series of physiological changes that mitigate the effects of hypobaric hypoxia and the resultant hypoxaemia. Whilst optimal acclimatisation may take weeks, or even months, to achieve, the initial acclimatisation process occurring in the first few days after ascent is usually enough to be protective against altitude sickness. The recommended ascent rate is no more than 300-500m ascent per day – sleeping altitude – with a rest day every 1000m or every 3-4 days. 36  Prophylaxis Acclimatisation