iHerp Australia Issue 14 | Page 43

In the wake of all these changes and additions, it seemed both an interesting and necessary exercise to review the relationships within the V. prasinus group. The natural distribution area of all members of this complex is limited to New Guinea and the surrounding islands (the only exception being V. keithhornei, which is located on the mainland of Australia), so it is entirely reasonable to suspect that genetic studies would be valuable to cast some light upon the respective phylogeny. ‘All species are very similar and differ almost only in colour.’ Indeed, since all species are phenotypically very similar and differ (almost) only in colour, Ziegler et al (2007) sought further evidence based on a DNA analysis. Unfortunately, not all the described taxa were available to them at that time. Several years ago now, a larger number of animals were confiscated by German Customs, so we took the opportunity to obtain blood samples from them all. The species were determined according to purely external characteristics, DNA was extracted from the blood samples and then was subsequently sequenced (Eidenmüller et al, 2017). In order to be able to incorporate the results of the previous study (Ziegler et al, 2007) into this new analysis, the same DNA fragment was sequenced and evaluated. Unfortunately, no usable DNA could be extracted from the type specimen of V. telenesetes. Above: V. kordensis. Left: V. beccarii. These were noted by Mertens in 1942 as the sole known subspecies of V. prasinus, but have since been raised to species status. All images courtesy of Bernd Eidenmüller unless otherwise noted.