The Journal
VEGF-D and VEGFR-3 that are intimately involved in oral cancer invasion and metastasis , was simultaneously down regulated significantly as determined by quantitative realt i m e R T - P C R , W e s t e r n b l o t a n d immunohistochemistry . archived oral mucosal tissues with cancerous and precancerous histopathology , including that of mild epithelial dysplasia ( 8 ). Thus , Bmi-1 expression occurs at a very early stage in oral carcinogenesis . It has been found that the endogenous knocking down of Bmi-1 in actively
Fig : Quantitative real-time RT-PCR analysis . The mRNA levels of u-PAR , u-PA and other metastasis-related genes including MMP-2 , MMP-9 , VEGF-C , VEGF-D and VEGFR-3 in Mock , EV , pUC and pU groups are shown in bars values were obtained by real time RT- PCR analysis using GAPDH transcripts for the normalization ( zhou et al . )
Down regulation of Bmi-1 protein in the cells of oral squamous cell carcinomas ( OSCC ) by RNAi
Bmi-1 is a polycomb group protein that was identified as c-myc cooperating oncogene in murine lymphomagenesis . In a recent study it has been determine the role of Bmi-1 in human oral carcinogenesis . Bmi-1 protein and RNA expression levels were markedly enhanced in the cells of oral squamous cell carcinomas ( OSCC ) compared with that of normal human oral keratinocytes ( NHOK ). Enhanced-Bmi-1 expression was also detected in situ in the
proliferating SCC4 cells and NHOK by RNA interference severely retards the division of the cells of OSCC .
The RNA interference of amino acid transporter LAT1 inhibits the growth of KB human oral cancer cells
Amino acid transporters are essential for growth and proliferation in all living cells . Among the amino acid transporters , the system L amino acid transporters are the major nutrient transport system responsible for the Na + - independent transport of neutral amino acids ,
35 Vol . 14 No . 1 Jan-Apr 2018