iDentistry The Journal identistry_may_aug2019 | 页面 5

*Dr. Anita Mehta
**Dr. Nitin Khuller
***Dr. Ramandeep Singh Brar
****Dr. Davinderjit Kaur Shergill
The Journal
Comparison of clinical methods of diagnosis and
monitoring of chronic inflammatory periodontal
disease with neutral protease and BANA hydrolase
Perioscan kit needs plaque sample to detect the presence of enzymes which are capable of
degrading N-benzoyl-DL-arginine-2-naphthylamide (BANA) from the relatively few anaerobic
periodontal pathogens, whereas Periocheck analyzes the presence of neutral proteases in the
gingival crevicular fluid.
Aim
The aim of this study was to compare the
traditional clinical methods of detecting
periodontal disease and to monitor with
Perioscan and Periocheck test kits to reflect
the response to initial therapy. 30 patients with
moderately severe chronic periodontitis were
examined before and after the course of oral
prophylaxis and root planing in 4 appointments.
Test assays and clinical measurements were
collected at 5 diseased sites and 3 healthy sites
in each subject. Complete data from 240 sites
were available for statistical analysis.
At baseline, Perioscan had a sensitivity of 97%
and a specificity of 54% and Periocheck had a
sensitivity of 87% and a specificity of 58% when
related to the clinical diagnosis. The
conventional method is by using periodontal
probe with millimetre gradations.
Periocheck test kit is a rapid chair side test kit to
detect the presence of neutral proteases
(Bowers & Zahradnik 1989). The presence of
neutral proteases enzymes implicated in
collagen breakdown. (Eley & Cox 1995).
Gingival Crevicular fluid is collected on the filter
paper strips and these are placed on a collagen
dye labelled gel matrix. Soluble dye labelled
fragments of collagen are formed from the
reaction of neutral proteases with the gel and
these diffuse onto the sample strip which turns
the colour of the paper to blue. The intensity and
quantity of the colour reaction is compared to a
standard colour chart and is related to the level
of neutral protease activity originally present in
the crevicular fluid sample.
The aim of this study was to evaluate the ability
of two diagnostic test kits to detect existing
periodontal disease
Material and Methods
After obtaining the informed consent from
patients thirty subjects (18 females,12 males) of
30-65 years of age and mean age was 36.6
years with moderate to severe periodontitis
were selected for this study. Those patients who
had recently received periodontal treatment,
recently taken antibiotics, or
had a systemic medical condition likely to
influence periodontal breakdown were
excluded from the study.
Clinical Measurements
Clinical variables were recorded and the
diagnostic tests performed at eight sites (5
diseased and 3 healthy) in each patients at
baseline and after a course of initial periodontal
treatment. Each patient with pocket depth of 5
mm deep or greater had at least 5 sites
* Associate Professor, Department of Periodontology & Implantology, D.I.R.D.S, Affiliated with B.F.U.H.S, Faridkot, Punjab, India
** Prof and HOD, Department of Periodontology & Implantology, D.I.R.D.S, Affiliated with B.F.U.H.S, Faridkot, Punjab, India
*** Professor, Department of Oral surgery, D.I.R.D.S, Affiliated with B.F.U.H.S, Faridkot, Punjab, India
****Assistant Professor, Department of Prosthodontics, Maharaja Ganga Singh Dental College and Research Centre, Sri Ganganagar
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Vol. 15
No. 2
May-Aug 2019