IASLC Sept 2013 | Page 49

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IASLC ATLAS OF ALK TESTING IN LUNG CANCER

Table 5. Comparison of Antibody Clones Used with IHC
Study Takeuchi et al., 2009 No. of Antibody Specimens Clone +
(% of ALK )

Antigen Retrieval Target Retrieval Solution (pH 9.0) (for all)

Detection/ Amplification System iAEP

Scoring 0 vs. + (for all)

Standard RT-PCR

IHC Sensitivity 100%

IHC Specificity 100%

(vs. Standard) (vs. Standard)

21 (52%)

5A4

5A4 ALK1 ALK1 SP8 SP8 Rodig et al., 2009 239 (4%) ALK1 ALK1 MinoKenudson et al., 2010 37 (59%) ALK1 EDTA in pressure cooker (for both) EDTA in pressure cooker (for both)

EnVision+ iAEP EnVision+ iAEP EnVision+ EnVision+ Tyramide amplification EnVision+ (for both) Score >2.7 on image analysis is positive 0 vs. + (for all) FISH 0 vs. + (for both) FISH

27% 100% 9% 20% 100% 40% 80% 67%

100% 100% 100% 100% 18% 100% 100% 97%

D5F3 Murakami et al., 2011 361a (5%) ALK1 Target Retrieval Solution (pH 9.0) (for all) ABC (no enhancement) EnVision FLEX+ EnVision FLEX+ Conklin et al., 2013 377 (3%) 5A4 According to manufacturer’s instructions (for all) iAEP 0, 1+, 2+, or 3+ (for all) FISH RT-PCR/ FISH

100% 81%

99% 100%

5A4

100% 100% 100%

100% 99.7% 62.5%

ALK1 ALK1 5A4 D5F3 Selinger et al., 2013 594 (11%) ALK1 Buffer (pH 9.0) in pressure cooker According to manufacturer’s instructions According to manufacturer’s instructions

EnVision FLEX ADVANCE ADVANCE ADVANCE EnVision FLEX+ ultraView and ultraView Amplification OptiView and OptiView Amplification 0, 1+, 2+, or 3+ (for all) FISH

66% 66% 100% 100% 100%

100% 87.5% 87.5% 75% 99%

5A4

100%

98%

D5F3

100%

99%

a

The number of specimens for testing with the D5F3 antibody was 356.

SP8 is a product of Abcam. Target Retrieval Solution, buffer, EnVision, EnVision FLEX+, and ADVANCE are products of Dako.. ultraView (Universal DAB Detection Kit), OptiView (DAB R?]X?[??]
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