32
IASLC ATLAS OF ALK TESTING IN LUNG CANCER
Table 2. Immunostaining Conditions Using Commercially Available ALK Antibodies in Selected Published Studies in which Commercially Available Kits Were Not Used
Study Yi et al., 2011 Antibody ALK1 ALK1 D5F3 D5F3 D5F3 5A4 5A4 5A4 5A4 5A4 Antigen Retrieval EDTA, pH 8.0, 30 min in PT Link EDTA, pH 8.0, in pressure cooker Heat mediated with BenchMark XT Standard on BenchMark XT CC1 solution, 100ºC, 20 min Target Retrieval Solution, pH 9.0, 97°C, 40 min CC1 solution with EDTA, pH 8.4, 1 hr CC1 solution, 100°C, 20 min Citrate buffer, pH 6.0, in pressure cooker, 122ºC, 30-45 min Citrate buffer, pH 6.0, in microwave, 95ºC, 30 min CC1 solution, 95ºC, 30 min Dilution 1:100 Incubatio