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IASLC ATLAS OF ALK TESTING IN LUNG CANCER
A
B
Figure 2. An adenocarcinoma that is strongly ALK positive. A: H & E-stained slide. B: ALK IHC with the D5F3 antibody and tyramide amplification shows intense cytoplasmic staining of ALK gene product (magnification, x40).
to detect with the ALK1 antibody, which is used to diagnose anaplastic large cell lymphoma (ALCL), as the protein expression is generally lower in NSCLC (Mino-Kenudson 2010). To overcome this issue, several technical steps have been introduced, including antigen retrieval, use of a primary antibody with higher affinity and at a sufficiently high concentration, strong signal amplification steps (e.g., with a tyramide cascade and intercalation of an antibody-enhanced polymer), an H]?[?Y[????[[?X??Y\?
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