Hooo-Hooo Hooo-Hooo Volume 11 Nr 4 - Page 18

WildLife Group of the SAVA and ischemic necrosis in conjunction with the demonstration of eosinophilic intracytoplasmic, viral inclusion bodies in keratinocytes of the epidermis and follicular epithelium as well as macrophages, endothelial cells, pericytes, acinar and ductal epithelial cells of the mucous and serous glands, and skeletal and smooth muscle cells. Immunohistochemistry enables detection of LSDV within histological sections and facilitates demonstration of the virus within histological lesions enabling definitive confirmation of the diagnosis (figure 3 and figure 4). Figure 4: Springbok skin – Lumpy skin disease IHC staining. Note the positive labelling of virus in sebaceous glands, macrophages and smooth muscle cells (asterix) Prevention and Control The cornerstone of prevention and control in cattle revolves around implementation of an effective vaccination program. “Neethling” strain vaccines as well as of live attenuated Sheeppox or goatpox vaccines (utilizing cross protection), have effectively been used to control LSD in cattle. There is currently no data available for the use of these vaccines in springbok. Vector control appears to have minimal effect on preventing disease. References Figure 3: Springbok skin – Lumpy skin disease IHC stain. Note the ballooning degeneration of the epidermis and follicular epithelium with strong, dark brown positive labelling of viral antigen including the intra-cytoplasmic inclusions in epidermal and follicular keratinocytes, and dermal macrophages (arrows) 18 1. Coetzer JAW & Tuppurainen E. Lumpy Skin Disease. http://www. afrivip.org/sites/default/files/02_lsd_eeva_epidemiology.pdf 2. World Organization for Animal Health (2017) – Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. OIE, Paris. 3. Jubb & Kennedy (2016). Pathology of Domestic Animals 6th edn.