Healthcare Hygiene magazine November 2019 | Page 21
Manufacturing and Regulatory/Compliance
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one of the newer ACs. Although gastroscopes were found
to be more highly contaminated than colonoscopes, they
were used for fewer procedures than colonoscopes. Every
endoscope had <10 CFU except one intervention AC with
15 CFU. Positive control samples were highly contami-
nated (ATP, 4,831 RLU; protein, 29 µg/mL; and microbial
cultures, >600 CFU). Negative control samples had low
ATP levels (9 RLU), negative protein tests (0 µg/mL), and no
microbial growth.
Technicians conducted ATP tests after manual cleaning
for all intervention endoscopes. Post-cleaning benchmarks
(<200 RLU) were met during 301 of 304 (99 percent) colo-
noscope encounters (mean, 17 RLU and median, 11 RLU),
and during 69 of 143 (48 percent) gastroscope encounters
(mean, 571 RLU and median, 214 RLU). In 16 (11 percent)
gastroscope encounters, the ATP levels were still high after
double manual cleaning and 2 cycles of cleaning and HLD
in the AER.
The take-home message from this study is that the
researchers found microbial growth in samples from 60
percent of endoscopes, and that residual fluid was found
in most endoscopes, which suggests insufficient drying
methods that can foster the growth of bacteria and fungi.
As the researchers emphasized, “This study demonstrated
that more rigorous reprocessing practices may not be suffi-
cient to ensure that patient-ready endoscopes are free from
residual contamination, particularly when the endoscope has
defects that could harbor organic debris and biofilm. Visual
inspection and routine monitoring for biochemical markers
evidence-
based
practices
Scientific R&D
were higher for gastroscopes than colonoscopes. Eighty-
five percent of endoscopes required repair due to findings.
Surprisingly, the researchers found that more rigorous
reprocessing was not consistently effective. Seven-day incu-
bation allowed identification of slow-growing microbes. The
researchers say their findings bolster the need for routine
visual inspection and cleaning verification tests recommended
in new reprocessing guidelines.
During the final assessment, researchers had observed
discoloration, scaly deposits, debris, scratches, and dents
on external surfaces. Gastroscope insertion tubes were
commonly stained yellow or orange and buckling was also
observed. Irregularities were often found on distal ends.
Borescope examinations revealed numerous irregularities,
including discoloration, scratches, and filaments of debris
protruding into channels. Researchers observed fluid in 19
of 20 (95 percent) patient-ready endoscopes, which were
stored vertically after reprocessing.
Researchers tested every endoscope in use at the final
assessment and found a similar proportion of endoscopes
in each group exceeded the post-cleaning benchmarks for
ATP (20 percent control and 30 percent intervention) and
protein (20 percent control and 20 percent intervention).
Overall, more gastroscopes exceeded the ATP benchmark (67
percent gastroscope and 7 percent colonoscope; but there
was no difference in protein levels (17 percent gastroscope
and 21 percent colonoscope). There were no differences in
cleaning effectiveness by endoscope age or use history, and
the highest post-HLD microbial colony count was found in
Infection
Prevention
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h ealthcarehygienemagazine
www.healthcarehygienemagazine.com
www.healthcarehygienemagazine.com • november 2019
Announcing our Annual Meeting!
January 22-23, 2020
This two-day event brings together a diverse
group of healthcare professionals and all of
the Institute’s Initiative Groups for face-to-face
meetings.
For the first time, day two will offer educational
sessions open to healthcare stakeholders.
Visit our website to learn more about our
2020 event, including:
• Training for healthcare professionals
• Our new Certification & Standards program
healthcaresurfacesinstitute.org
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