Synchrotron Radiation micro-computed tomography ( SR-μCT ) is based on high flux X-ray in a particle accelerator . The acquired images have a high resolution and allow simultaneous visualization of the specimen ’ s 3-D microstructure and quantitative analysis of the segments , along with their densities . With SR-μCT the intrinsic limitations of traditional histomorphometry microcopy , e . g ., the 2-D nature of images or specimen defects during sectioning , are surmounted . It is also possible to study the bone regeneration dynamic , including the |
ratio and distribution of new and substitute bone and their interfaces at different times , which is not possible using conventional µ CT or historical histomorphometry .
In our study , sinus floor augmentation was performed using the lateral approach and Geistlich Bio-Oss ® .¹ After 6-months of uneventful healing , the bone biopsy specimens were collected during implant site preparation and cut into a cylindrical shape for tomography image acquisition ( 3 mm long and
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2 mm diameter ). After reconstruction of 3-D images from the tomography slices ( Fig . 1A ), the true structure of regenerated bone was revealed ( Fig . 1B & C ). Quantitative analysis indicated the volume fractions of new bone , Geistlich Bio-Oss ® and woven bone were 29.44 %, 13.39 %, 13.29 %, respectively .
Reference
1 Seo SJ , Kim YG .: J Synchrotron Radiat . 2020 1 ;
27 ( Pt 1 ): 199-206 .
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Histology : Yong-Gun Kim |
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30 GEISTLICH NEWS 1-2022 |