Geistlich News No.1 2022 | Page 30

Image analysis

Navigation inside the 3-D pore structure of regenerated bone

Yong-Gun Kim | Republic of Korea Department of Periodontology , School of Dentistry , Kyungpook National University , South Korea
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Fig . 1 : | A High contrast micro-tomography slices allow segmentation of the newly formed bone , Geistlich Bio-Oss ® , and woven bone – pixel size 1.8 µ m . | B & C 3-D reconstructed image of sample : purple showing the woven bone in the interface of remaining Geistlich Bio-Oss ® ( white ) and the newly formed bone ( red ).
Synchrotron Radiation micro-computed tomography ( SR-μCT ) is based on high flux X-ray in a particle accelerator . The acquired images have a high resolution and allow simultaneous visualization of the specimen ’ s 3-D microstructure and quantitative analysis of the segments , along with their densities . With SR-μCT the intrinsic limitations of traditional histomorphometry microcopy , e . g ., the 2-D nature of images or specimen defects during sectioning , are surmounted . It is also possible to study the bone regeneration dynamic , including the
ratio and distribution of new and substitute bone and their interfaces at different times , which is not possible using conventional µ CT or historical histomorphometry .
In our study , sinus floor augmentation was performed using the lateral approach and Geistlich Bio-Oss ® .¹ After 6-months of uneventful healing , the bone biopsy specimens were collected during implant site preparation and cut into a cylindrical shape for tomography image acquisition ( 3 mm long and
2 mm diameter ). After reconstruction of 3-D images from the tomography slices ( Fig . 1A ), the true structure of regenerated bone was revealed ( Fig . 1B & C ). Quantitative analysis indicated the volume fractions of new bone , Geistlich Bio-Oss ® and woven bone were 29.44 %, 13.39 %, 13.29 %, respectively .
Reference
1 Seo SJ , Kim YG .: J Synchrotron Radiat . 2020 1 ;
27 ( Pt 1 ): 199-206 .
Histology : Yong-Gun Kim
30 GEISTLICH NEWS 1-2022