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Publications
Hugo Calixto Fonseca doctoral student in Food Science. He recently published the article “Probiotic Properties of Lactobacilli and Their Ability to Inhibit the Adhesion of Enteropathogenic Bacteria to Caco-2 and HT-29 Cells.”, In Probiotics and Antimicrobial Proteins. The work evaluated the probiotic properties of lactic acid bacteria using resistance, safety and functional tests. All strains had high (≥ 80.0%) survival rates in gastric and intestinal juices. Among them, Lactobacillus brevis CCMA 1284, L. plantarum CCMA 0743 and L. plantarum CCMA 0359 exhibited greater hydrophobicity (95.33, 96.06 and 80.02%, respectively), while L. paracasei CCMA 0504 and L. paracasei CCMA 0505 had the highest self-aggregation values (45.36 and 52.66%, respectively). However, these last two strains were positive for the DNAse test, which is a safety concern. CCMA 0359 and CCMA 1284 strains showed no antimicrobial activity, while strain CCMA 0505 showed a higher percentage of adherence (4.75%) to Caco-2 cells. In simulated competition and exclusion assays, strain CCMA 0743 was able to reduce Salmonella adhesion to both cells (Caco-2 and HT-29), but only strains CCMA 0743 and CCMA 0505 inhibited Escherichia coli adhesion to HT29 cells in the competition assay. Demonstrating that the evaluated strain presented itself as an excellent candidate for the use of probiotics.
https://doi.org/10.1007/s12602-020-09659-2