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Further sources: References English FP, Nutting WB (1981), ‘Demodicosis of ophthalmic concern’ Am J Ophthalmol, 91:362–372. 1  Lee SH, Chun YS, Kim JH, et al. (2010), ‘The relationship between demodex and ocular discomfort’, Invest Ophthalmol Vis Sci. 51:2906–2911 2  Spickett SG (1961), ‘Studies on Demodex folliculorum’, Parasitology, May, 51:181-192. 3  www.bbc.co.uk/earth/story/20150508- these-mites-live-on-your-face 4  Turk M, Ozturk I, Sener AG, et al. (2007), ‘Comparison of incidence of Demodex folliculorum on the eyelash follicule in normal people and blepharitis patients’, Turkiye Parazitol Derg. 31:296–297. 5  Liu J, Sheha H, Tseng SC (2010), ‘Pathogenic role of Demodex mites in blepharitis’, Curr Opin Allergy Clin Immunol, 10:505-10 6  Despite the patient exiting the first treatment session with perfectly clean- to-the-base lashes, the CD’s return to the extent that they appear similar to the pre-treatment visit. As the treatment weeks advance, there is progressively less returning CD to deal with. Because TTO or T4O usage promotes outward migration of Demodex, the quick reformation of CD after opening the follicles is expected, as Demodex are being purged. With a decreasing active population of Demodex, the amount of CD being formed also reduces and this is conformational the treatment is working. We have noticed a proportional relationship between the amount of resurgent CD and the initial pre-treatment CD count. Innovative treatment Tea tree oil contains chemically sensitive substances. Several oil constituents oxidize on contact with air at room temperature, especially when there’s light, too. In this way, air greatly reduces the terpinen-4-ol content. What’s worse is that the gradual loss of active constituents is accompanied by a dramatic rise in the concentration of substances, such as p cymol, ascaridol and 1,2,4-trihydroxymenthane, which irritate the skin and can cause allergic reactions. One way out of this predicament is the molecular inclusion of T4O in a suitable cyclodextrin – a method that has proved effective for fragrances, vitamins, and other lipophilic substances. Cyclodextrins are ring-shaped sugar molecules comprising several interlinked glucose units. Each cyclodextrin molecule can house a lipophilic guest molecule in its cavity, and will release it again under suitable conditions. It is best to imagine a cyclodextrin molecule as a tiny safe in which an individual molecule is kept and protected against the influence of oxygen, light and heat. When necessary, the safe is opened 16 etCETera | June 2016 and the molecule emerges completely unchanged – as fresh as when it was put inside. The key to opening these molecular safes is moisture. For as long as the T4O remains enclosed in the cyclodextrin, it enjoys perfect protection – it can neither evaporate nor be altered chemically. The skin’s natural moisture and temperature are sufficient to release the T4O. The T4O thus reaches the skin in juvenile form. There are no skin irritating and sensitising oxidation and degradation products. Accelerated treatment To improve patient outcomes for severe cases, we have tried a slow release version of T40 entrapped in Cyclodextrin on four patients, to deliver 10% T4O active over 12 hours. The product particles are around 5000nm in size, which is far too large for deep follicular penetration and resemble talcum powder. Applied onto the eyelids and lashes before sleep it is absent upon wakening. Patients report no irritation whatsoever. We have observed a greater production of resurgent CD, which may imply a quicker evacuation of Demodex. Another agent, related to limonene and a by-product of the orange juice industry is also miticidal but has not yet been commercialised. There is scope for further product development. Currently, Demodex treatment is an arduous process for both practitioner and patient. The time burden, commitment required and commercial revenue lost in the disruption of the provision of spectacles and contact lenses makes this work unattractive for most practices. However the professional reward in curing a condition where others have failed is priceless and the patients are extremely grateful. About the author David Crystal transferred from an undergraduate degree course in Physics with Computing at Bradford University to study Optometry at Caledonian University Glasgow in 1980. He established Scotland’s first specialised dry and watery eye clinic, with routine punctal occlusion and tear duct syringing procedures. David gained his Post Graduate Diploma in Ocular Therapeutics in 2002, becoming the first of his kind in Scotland. He now occasionally facilitates workshops for Glasgow Caledonian University Ocular Therapeutics course. David is currently pursuing treatments that eradic ate Demodex; the most common indirect cause of evaporative dry eye. Outside of optometry, his interests are snowboarding and website SEO. He also created EyeDispense, Just Reading Test Types and Maddox Rod iPad apps. 7  heirkhah A, Casas V, Li W, et al. ‘Corneal K manifestations of ocular Demodex infestation’. English FP, Nutting WB (1981), ‘Demodicosis of ophthalmic concern’, Am J Ophthalmol, 91:362–372. 8  Bonnar E, Eustace P, Powell FC (1993), ‘The Demodex mite population in rosacea’, J Am Acad Dermatol. 28:443–448. 9  Huang Y, He H, Sheha H, Tseng SCG (2013), ‘Ocular demodicosis as a risk factor of pterygium recurrence’. Ophthalmology, 120:1341–1347. 10  Gao Y-Y, Di Pascuale MA, Li W, et al. (2005), ‘High prevalence of ocular Demodex in lashes with cylindrical dandruffs’. Invest Ophthalmol Vis Sci. 46:3089–3094. [PubMed] 11  Nichols KK, Foulks GN, Bron AJ, et al. (2011), ‘The inter- national workshop on meibomian gland dysfunc- tion: executive summary’ Invest Ophthalmol Vis Sci. 52(4):1922-9. 12  Gao Y-Y, Di Pascuale MA, Li W, et al. (2005), ‘High prevalence of ocular Demodex in lashes with cylindrical dandruffs’. Invest Ophthalmol Vis Sci. 46:3089–3094. [PubMed] 13  Tighe et al. (2013), ‘Terpinen-4-ol is the Most Active Ingredient of Tea Tree Oil to Kill Demodex Mites’ http://www.ncbi. nlm.nih.gov/pmc/articles/PMC3860352/ 14  Gao YY, Xu DL, Huang lJ, Wang R, Tseng SC. (2012), ‘Treatment of ocular itching associated with ocular demodicosis by 5% tea tree oil ointment’, Cornea. Jan. 31(1):14-7. [Medline]. 15  Simpson et al. (2004) ‘Prevalence of botanical extract allergy in patients with contact dermatitis’, Dermatitis 15:67-72. 16  Kheirkhah A, Blanco G, Casas V, Tseng SC (2007), ‘Fluorescein dye improves microscopic evaluation and counting of demodex in blepharitis with cylindrical dandruff’, Cornea. Jul. 26(6):697-700. [Medline]. 17  English FP, Cohn D, Groeneveld ER. (1985), ‘Demodectic mites and chalazion. Am J Ophthalmol’, 100:482–483. [PubMed] Kheirkhah A, Casas V, Li W, et al. (2007), ‘Corneal manifestations of ocular Demodex infestation’, Am J Ophthalmol. 143:743–749. Kim J, Chun Y, Kim J. (2011), ‘Clinical and immunological responses in ocular demodecosis’, J Korean Med. 26:1231–1237. Knop E, Knop N, Millar T, et al. (2011), ‘The international workshop on meibomian gland dysfunction: report of the subcommittee on anatomy, physiology, and pathophysi- ology of the meibomian gland’, Invest Ophthalmol Vis Sci. 52(4):1938-78. Koksal M, Kargi S, Taysi BN, Ugurbas SH. (2003), ‘A rare agent of chalazion: demodectic mites’, Can J Ophthalmol. 38:605–606. [PubMed] Liang L, Safran S, Gao Y, et al. (2010), ‘Ocular demodicosis as a potential cause of pediatric blepharoconjunctivitis’, Cornea. 29:1386–1391. Obata H. (2002), ‘Anatomy and histopathology of human meibomian gland’, Cornea. 21(7 Suppl):S70-4 24. Wertz PW (2009), ‘Human synthetic sebum formulation and stability under conditions of use and storage’, Int J Cosmet Sci. Feb, 31(1):21-25 David Crystal can be contacted at www.crystaloptometry.co.uk June 2016 | etCETera 17