EQUINE | Abstracts
EQUINE | Abstracts
( P < 0.001 ). Neutrophil morphology grade was significantly greater at T24 , T48 and T72 , compared to T0 , for both 4 ° C and 22 ° C ( P < 0.001 ). For 9 samples , the diagnostic interpretation changed over time . Specificity and positive predictive value at each analysis point was 100 %; however , sensitivity and negative predictive value decreased with greater storage duration and temperature .
CONCLUSIONS : Alterations in the TNCC and neutrophil morphology of body fluid samples occur when analysis is delayed , especially with higher storage temperatures , and may influence interpretation and clinical decision making . Body fluid samples should be analysed as soon as possible after collection to minimise pre-analytical errors from storage .
The Effects of Mepivacaine Hydrochloride on Antimicrobial Activity and Mechanical Nociceptive Threshold During Amikacin Sulfate Regional Limb Perfusion in the Horse .
Colbath AC , et al . Vet Surg . 2016 .
Abstract
OBJECTIVE : To determine the effect of intravenous regional limb perfusion ( IVRLP ) with a combination of mepivacaine hydrochloride and amikacin sulfate on synovial fluid amikacin sulfate concentration , antimicrobial activity , and mechanical nociceptive threshold ( MNT ). mL saline ). Opposite treatments were repeated after a 24 hour wash-out period . Amikacin concentration and antimicrobial activity were determined for synovial fluid from middle carpal joints at tourniquet removal and 30 minutes following . Zone of inhibition was determined for Staphylococcus aureus and Escherichia coli . MNT was determined at 3 dorsal metacarpal locations prior to and after sedation , after Esmarch tourniquet application , and 30 minutes after IVRLP prior to and after tourniquet removal .
RESULTS : Two limbs from each treatment group were removed because of undetectable amikacin concentrations for a total of 14 data sets analyzed . Synovial fluid amikacin concentrations and zone of inhibition were not significantly different between treatments at any time point . MNT were significantly increased 30 minutes after IVRLP prior to and following tourniquet removal using amikacin and mepivacaine ( median , range ; 40.0 µ g / mL , 38.7-40.0 and 40.0 , 25.8-40.0 , respectively ) compared to amikacin alone ( 19.5 µ g / mL , 18.7-25.6 and 15.3 , 13.2-20.5 , respectively ).
CONCLUSION : Addition of mepivacaine to amikacin for IVRLP in the horse as a means of providing analgesia without decreasing antimicrobial activity .
STUDY DESIGN : Experimental study . ANIMALS : Healthy adult horses ( n = 9 ).
METHODS : One IVRLP treatment was randomly administered by cephalic vein of each limb : amikacin alone ( 1 g amikacin in 60 mL saline ) or amikacin with mepivacaine ( 1 g amikacin and 500 mg mepivacaine in 60
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