Equine Health Update EHU Vol 21 Issue 02 | Page 20

EQUINE | Abstracts Prospective, randomised clinical trial of four different presurgical hand antiseptic techniques in equine surgery N. M. Biermann, J. T. McClure, J. Sanchez, M. Saab, A. J. Doyle Background Results Objectives Main limitations Study design Conclusions Currently, the World Health Organization recommends the use of alcohol‐based hand rubs (ABR) for surgical hand preparation in human surgery. When disinfecting soaps are used, a rubbing technique causes less skin irritation than brush scrubbing. Based on a recent survey, most equine surgeons still use disinfecting soap. The efficacy of scrubbing vs. rubbing and the use of sole ABR compared with chlorhexidine (CHx)‐ based products has not been evaluated in the equine surgical setting. To compare four surgical hand antisepsis techniques in equine surgery for reduction of aerobic bacterial counts from pre‐ to post‐preparation (immediate efficacy) and at the end of surgery (sustained efficacy). Randomised, prospective clinical trial. Methods A 4% CHx‐based product applied with either a scrub or rub technique, one sole ABR (IPO; 30% 1‐ propanol and 45% 2‐propanol) and one CHx/alcohol‐ combination (CHx/ET; 1% CHx and 61% ethanol) product both applied with a rub technique were evaluated. Samples were collected by glove juice technique and cultured on 3M™ Petrifilm plates and counted using a 3M™ Petrifilm plate reader. 20 Immediate mean bacterial log10 colony forming unit (CFU) reduction was 2.4 for CHx‐scrub, 2.8 for CHx‐ rub, 3.1 for CHx/ET and 2.1 for IPO. CHx/ET resulted in significantly lower bacterial counts than CHx‐scrub (P<0.005) and IPO (P<0.001) while CHx‐rub resulted in significantly lower counts than IPO (P<0.001). At the end of surgery bacterial counts were the lowest for CHx‐ rub, significantly lower than CHx/ET (P<0.001) and IPO (P<0.001). There was no difference between CHx‐rub and ‐scrub techniques (P = 0.7). Bacterial counts were used as the outcome measure rather than prevalence of surgical site infection, and the effect of hand preparation on skin health was not assessed. ABR did not decrease bacterial log10CFU counts more effectively than CHx products. When using CHx soaps in the equine setting, hand‐rub is as effective as a hand‐scrub‐technique. • Equine Health Update •