Enzyme
Assays
Spectrophotometric assays observe change
in the absorbance of light between
products and reactants
Radiometric assays involve the
incorporation or release of radioactivity to
measure the amount of product made over
time.
Enzyme assays are laboratory procedures that measure the rate of
enzyme reactions. Since enzymes are not consumed by the reactions they
catalyse, enzyme assays usually follow changes in the concentration of
either substrates or products to measure the rate of reaction. There are
many methods of measurement.
The most sensitive enzyme assays use
lasers focused through a microscope to
observe changes in single enzyme
molecules as they catalyse their reactions.
Creative Enzymes Inc.
www.creative-enzymes.com