Non-Michaelis–Menten Kinetics
Some enzymes produce a sigmoid v by [S] plot, which often indicates cooperative
binding of substrate to the active site. This means that the binding of one substrate
molecule affects the binding of subsequent substrate molecules.
This behavior is most common in Positive cooperativity occurs when
multimeric enzymes with several binding of the first substrate molecule
interacting active sites. Here, the increases the affinity of the other active
mechanism of cooperation is similar sites for substrate. Negative
to that of hemoglobin, with binding of cooperativity occurs when binding of the
substrate to one active site altering first substrate decreases the affinity of
the affinity of the other active sites for the enzyme for other substrate
substrate molecules. molecules.
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