Ternary-complex Mechanisms
In these enzymes, both substrates bind to the enzyme at the same time to produce an EAB ternary complex.
The order of binding can either be random (in a random mechanism) or substrates have to bind in a
particular sequence (in an ordered mechanism). When a set of v by [S] curves (fixed A, varying B) from an
enzyme with a ternary-complex mechanism are plotted in a Lineweaver–Burk plot, the set of lines produced
will intersect.
Enzymes with ternary-complex mechanisms include glutathione S-transferase, dihydrofolate reductase and
DNA polymerase.
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