Student Poster Presentation #13 (Session 2)
Heterotypic Cell-cell Interactions of Human Induced Pluripotent Stem
Cells and Human Mesenchymal Stem Cells for Neural Differentiation
Liqing Song 1 , Ang-Chen Tsai 1 , Xuegang Yuan 1 , Julie Bejoy 1 ,
Sébastien Sart 2 , Teng Ma 1 , Yan Li 1
1. Department of Chemical and Biomedical Engineering; FAMU-FSU College of Engineering; Florida State
University; Tallahassee, FL USA. 2. Hydrodynamics Laboratory (LadHyX) - Department of Mechanics;
Ecole Polytechnique; CNRS-UMR7646; 91128 Palaiseau; France.
Abstract
Organ buds, the condensed 3-D tissues emerged at the early stage of organogenesis, are a promising
transplantation entity to regenerate functional and vascularized organs. However, complex heterotypic
interactions of different cell types, such as human mesenchymal stem cells (hMSCs) and human induced
pluripotent stem cell (hiPSC) derived neural progenitors, and their secretory activities during neurogenesis have
not been well understood. The objective of this study is to investigate the impact of the composition and structure
of 3-D hybrid spheroids of hiPSCs and hMSCs on neural differentiation and the secretion of extracellular matrices
and trophic factors in vitro. The hybrid spheroids were formed at different hiPSC:hMSC ratios (100:0, 75:25,
50:50, 25:75, 0:100) using direct mixing and pre-
hiPSC aggregation method, which generated
dynamic spheroid structure.
The cellular
organization,
proliferation,
neural
marker
expression, the secretion of extracellular matrix
proteins and the cytokines were characterized. The
incorporation of MSCs upregulated the neural
marker expression, matrix remodeling proteins and
the secretion of transforming growth factor (TGF)-
β1 and prostaglandin E2. This study indicates that
the composition and structure of hiPSC-MSC
Figure 1. Spatial cell distribution of hiPSC-hMSC
spheroids
differentially
influence
neural
spheroids formed by direct mixing and pre-hiPSC
differentiation and trophic factor and matrix
aggregation at different culture time.
secretion due to the modulation of heterotypic cell-
cell interactions.
Reference
1. Takebe T, M Enomura, E Yoshizawa, M Kimura, H Koike, Y Ueno, T Matsuzaki, T Yamazaki, T Toyohara, K
Osafune, H Nakauchi, HY Yoshikawa and H Taniguchi. (2015). Vascularized and complex organ buds from diverse
tissues via mesenchymal cell-driven condensation. Cell Stem Cell 16:556-65.
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