Poster Presentation 9
Catalase-Laden Microdevices for Cell-Mediated Enzyme Delivery
Junfei Xia1, Zhibin Wang1, Yuanwei Yan1, Zhijian Cheng2, Li Sun2, Yan Li1, Yi Ren2,
Jingjiao Guan1,*
1
Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University
2
Department of Biomedical Sciences, College of Medicine, Florida State University
Abstract
Enzymes have been used as drugs to treat a wide variety of human diseases and traumas. However, therapeutic
utility of free enzymes is generally impeded by short circulation time, lack of targeting ability, immunogenicity,
and inability to cross certain biological barriers. Various strategies have been developed to overcome these
limitations. Among them is cell-mediated drug delivery, which is featured by integrating drugs with live cells
and taking advantage of the unique capabilities of the cells to achieve controlled drug delivery. We developed
simple and inexpensive methods based on soft lithography and layer-by-layer (LbL) assembly for fabricating
disk-shaped microparticles termed microdevices and generating the cell-microdevice complexes. [1, 2] We
herein applied these methods to the fabrication of enzyme-laden microdevices and corresponding cellmicrodevices complexes for the first time. We used catalase as a model enzyme in this study. It is a water
soluble protein with an isoelectric point of 5.8. It is thus negatively charged at pH 7. Poly(diallyldimethyl
ammonium chloride) (PDAC) was chosen here as a polycation to form a bilayer with catalase. Catalase in the
microdevices was catalytically active and active catalase was slowly released from the microdevices. The
catalase-laden microdevices were attached to the external surface of live suspension and adhesion cells
respectively to form cell-microdevice complexes. This technique is applicable to other therapeutic enzymes and
therapeutic cells, and thus promises to find clinical applications for treating various human diseases and
traumas.
References
1. Junfei Xia, Zhibin Wang, Danting Huang, Yuanwei Yan, Yan Li, Jingjiao Guan*,Asymmetric Biodegradable Microdevices