CANNAHealthcare Magazine Volume 5, 2nd Quarter, 2018 | Page 39

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In addition, we will derive suspension cultures from

cells of specific cannabis cultivars, for the production

of secondary metabolites such as terpenes and alkaloids.

Cell suspension cultures are the multiplication of cells

starting from single cells or small aggregates of cells using agitated liquid medium. Depending on the cell type being used, the growth medium can be experimentally optimized to produce the cell cultures better and faster. We hope to experiment with chemical triggers for the production of cannabinoids in the suspension culture. Overall, we hope to elucidate novel ways to produce cannabinoids of medical importance without growing entire cannabis plants. This would lead to a faster production of cannabinoids that is pesticide-free with less-space usage and less-energy usage.

We also want to position ourselves to take advantage of other techniques, which can improve the medical value of cannabis plant, but still require sterile tissue cultures of various cannabis cultivars. For example, in the future we would like to apply precision breeding techniques including targeted editing of the cannabis genome to enhance qualities such as the chemical profile, developmental and morphological attributes. Any genetic modification at the molecular level will require sterile plant/cell culture for the production of the final plant.

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In addition, we will derive suspension cultures from

cells of specific cannabis cultivars, for the production

of secondary metabolites such as terpenes and alkaloids.

Cell suspension cultures are the multiplication of cells

starting from single cells or small aggregates of cells using agitated liquid medium. Depending on the cell type being used, the growth medium can be experimentally optimized to produce the cell cultures better and faster. We hope to experiment with chemical triggers for the production of cannabinoids in the suspension culture. Overall, we hope to elucidate novel ways to produce cannabinoids of medical importance without growing entire cannabis plants. This would lead to a faster production of cannabinoids that is pesticide-free with less-space usage and less-energy usage.

We also want to position ourselves to take advantage of other techniques, which can improve the medical value of cannabis plant, but still require sterile tissue cultures of various cannabis cultivars. For example, in the future we would like to apply precision breeding techniques including targeted editing of the cannabis genome to enhance qualities such as the chemical profile, developmental and morphological attributes. Any genetic modification at the molecular level will require sterile plant/cell culture for the production of the final plant.