Human genes critical to immunity or inflammation also play role in HIV- 1 pathogenesis and have been of interest for genetic studies. Polymorphisms in the genes encoding cytokines, cytokine receptors have been associated with both resistance and disease progression kinases. More recently, the use of CRISPR / Cas9 genome editing approach has also confirmed host CD4 and CCR5 along with ALCAM( responsible for cell aggregation required for cell-to-cell HIV transmission), TPST2 and SCL35B2( facilitate CCR5 recognition) to be essential for HIV infection. These new generation host genetic
approaches are critical to functionally validate genome wide studies and identify therapeutic targets to control HIV infection. In conclusion, it is detrimental to undertake large cohort studies including individuals from multiple ethnic background and integrate different layers of data generated using multiple approaches.
siRNA transfection and shRNA transduction studies, minimal overlap across studies was investigated in genes potentially necessary for optimal viral replication. These investigations emphasize the necessity to improve precision of transcriptome analysis through added resolution of RNA-Seq. However, genes identified in two or more independent transfection screens belonged to nuclear pore machinery, components of NF-kB complex and key
Dr Partha P. Majumder is a Professor of Human Genetics in the Indian Statistical Institute, Kolkata, and the founder of the National Institute of Biomedical Genomics, Kalyani, West Bengal, where he is currently a Distinguished Professor. He is also a Sir J. C. Bose National Fellow. He has pioneered the development of many statistical methods in the analysis of human genome diversity and genetic epidemiology.
Dr Ankita Garg, ANKITA GARG is an Assistant Professor at the National Institute of Biomedical Genomics, Kalyani, West Bengal. She has over 12 years of experience in translational research in the field of infectious diseases and immunology. She is recipient of various national and international awards including Young Investigator Award from IAS-USA and Global Health Travel Award by Bill and Melinda Gates Foundation.
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