Biotech 2nd Edition Sample Ch. 4 Biotechnology Sample Ch. 4 | Page 3

3. Measure out the TRIS and EDTA, and add them to a 250-mL beaker. 4. Add 80 mL of deionized water and mix until the chemicals dissolve. Use pH paper to determine the pH. The desired pH is 8.0. If the pH is between 7.5 and 8.5, record the pH on the label and do no further pH adjustment. If the pH is not within \