RECENT RESEARCH
Conclusion : The findings of this study suggest that the M . splendens EO has cytotoxic compounds for the A549 lung cancer cells . Treatment with the EO decreased the colony formation and reduced the ability of lung cancer cells to migrate . Future studies may be used to isolate compounds from the EO for the study of lung cancer .
Bencze B , Temesfoi V , Das S , Papp H , Kaltenecker P , Kuczmog A , Jakab F , Kocsis B , Koszeqi T . Development of a novel , entirely herbal-based mouthwash effective . BMC Complementary Medicine and Therapies . 2023 ; 23 , Article number : 138 .
Background : Parallel to the growth of the oral healthcare market , there is a constantly increasing demand for natural products as well . Many customers prefer products that contain fewer toxic agents , therefore providing an environmentally friendly solution with the benefit of smaller risk to the user . Medieval and early modern medicinal knowledge might be useful when looking for natural , herbal-based components to develop modern products . Along with these considerations we created , tested , and compared an entirely natural mouthwash , named Herba Dei .
Methods : The manufacturing procedure was standardized , and the created tincture was evaluated by GC / MS analysis for active compounds , experimentally tested in cell-based cytotoxicity , salivary protein integrity , cell-free antioxidant activity , antibacterial and anti-viral assays , and compared with three market-leading mouthwashes .
Results : Our tincture did not show significant damage in the cytotoxicity assays to keratinocyte and Vero E6 cells and did not disrupt the low molecular weight salivary proteins . Its radical scavenging capacity surpassed that of two tested , partly natural , and synthetic mouthwashes , while its antibacterial activity was comparable to the tested products , or higher in the bacterial aerobic respiratory assay . The active compounds responsible for the effects include naturally occurring phenylpropanoids , terpenes , and terpenoids . Our mouthwash proved to be effective in vitro in lowering the copy number of SARS-CoV-2 in circumstances mimicking the salivary environment .
Conclusions : The developed product might be a useful tool to impede the transmission and spread of SARS-CoV-2 in interpersonal contact and aerosolgenerating conditions . Our mouthwash can help reduce the oral bacterial flora and has an antioxidant activity that facilitates wound healing and prevents adverse effects of smoke in the oral cavity .
Herbal medicine
Safari M , Asadi A , Aryaeian N , Huseini HF , Shidfar F , Jazayeri S , Malek M , Hosseini AF , Hamidi Z . The effects of melissa officinalis on depression and anxiety in type 2 diabetes patients with depression : a randomized double-blinded placebo-controlled clinical trial . BMC Complementary Medicine and Therapies . 2023 ; 23 , Article number : 140 .
Background : Depression is more common in diabetic patients , with a 1.5-fold increased risk of death . Melissa officinalis ( M . officinalis ) have antidiabetic and anti-depression activities . The study aimed to determine the efficacy of M . officinalis extract on depression , anxiety , and sleep quality in patients with type 2 diabetes with depressive symptoms .
Methods : In this double-blind clinical trial , 60 volunteer patients ( age range 20 – 65 years ) with type 2 diabetes mellitus with symptoms of depression were randomized into the intervention ( received 700 mg / day hydroalcoholic extract ; n = 30 ) or control group ( received 700 mg / day toasted flour ; n = 30 ). Dietary intake , physical activity , anthropometric indices , FBS ( Fasting blood sugar ), hs-CRP ( High-sensitivity C-reactiveprotein ), depression , anxiety , and sleep quality were determined at the beginning and end of the study . Depression and anxiety were assessed by Beck Depression Inventory-II ( BDI- II ) and Beck Anxiety Inventory ( BAI ), respectively ; sleep quality was evaluated using the Pittsburgh Sleep Quality Index ( PSQI ).
Results : Sixty participants received M . officinalis extract or placebo , of which 44 patients completed the 12- week double-blind clinical trial . After 12-week the mean change of depression and anxiety scores were statistically significant between the two groups ( p < 0.001 and p = 0.04 , respectively ), but no significant differences were observed in FBS , hs-CRP , anthropometric indices , sleep quality , and blood pressure . In the intervention group , there was a significant decrease in depression and anxiety severity ( p < 0.001 and p = 0.01 , respectively ) at the end of the study compared to the baseline .
Trial registration : All protocols in this study were followed in accordance with the Helsinki Declaration ( 1989 revision ). Ethical approval for this study was obtained from the Iran University of Medical Sciences Ethics committee ( IR . IUMS . FMD . REC 1396.9413468004 ; research . iums . ac . ir ). The study was registered at the Iranian Registry of Clinical Trials ( IRCT201709239472N16 ); Registration date : 09 / 10 / 2017 .
Campos MF , Baratto LC , Vidal VM , Nascimento IV , Gomes BA , Neto G , Olsen PC , Leo RR , Moreira LO . Bactericidal and anti-inflammatory effects of Moquilea tomentosa Benth . flavonoid-rich leaf extract . BMC Complementary Medicine and Therapies . 2023 ; 23 , Article number : 153 .
Background : Natural products are an important source of bioproducts with pharmacological properties . Here we investigate the components of leaves from M . tomentosa Benth . ( Fritsch ) ( Chrysobalanaceae ) and its effects on bacterial cell growth , biofilm production and macrophage activity .
Methods : The effect of the different leaf extracts against bacterial cell growth was performed using the microdilution method . The most active extract was analyzed by mass spectrometry , and its effect on bacterial biofilm production
JATMS | Winter 2023 | 101