Study . Multidisciplinary Respiratory Medicine , 2016 , 11 , 12 . The goal of this controlled observational study was to investigate the role of a homeopathicallyprepared medicine ( Anas barb ) in preventing respiratory tract infections ( RTIs ). The authors analysed data from 459 people suffering from RTI ' s , 248 of whom were treated with Anas , and 211 were not treated . All were followed up for at least 1 year , and up to a maximum of 10 years . A significant reduction in the frequency of onset of RTIs was found in both the homeopathic medicine group and the untreated group . The reduction in the mean number of RTI episodes during the period of observation versus the year before inclusion in the study was significantly greater in the homeopathictreated group than in untreated patients .
6 . Rossi E , et al . Homeopathic Therapy in Pediatric Atopic Diseases : Shortand Long-Term Results . Homeopathy , 2016 , 105 , 3 , 217-224 . An Italian team studied the outcomes of atopic diseases ( atopic dermatitis , atopic rhintis and asthma ) in 857 children treated with homeopathy at the Homeopathic Clinic of Lucca ( Italy ) and related long-term results after approximately an 8-year period . The results showed that 75.8 % of atopic children had moderate or major improvement ( 67.1 % with asthma as the primary disease ; 84.2 % rhinitis ; 84.2 % dermatitis ). At re-evaluation after 5-10 years , complete remission of atopic symptoms was obtained in 70.1 % of the children : 84.2 % in dermatitis ; 48.1 % in allergic rhinitis ; 71.4 % in asthma . Children with two or three atopic diseases at the first visit were completely cured in 40 % of cases .
In-Vitro Studies
1 . Bigagli E , et al . Effects of Extreme Dilutions of Apis mellifica Preparations on Gene Expression Profiles of Human Cells . Dose-Response , January- March , 2016 , 1-7 . Using whole-genome transcriptomic analysis , the authors of this work investigated the effects of a wide range of homeopathic preparations of Apis mellifica on the gene expression profiles of human cells . RWPE-1 cells , a nonneoplastic adult human epithelial prostate cell line , were exposed to Apis preparations ( 3C , 5C , 7C , 9C , 12C , 15C , and 30C ) or to controls for 24 hours . Non-exposed cells were also checked for gene expression variations . The results showed that even the most diluted solutions retained the ability to trigger significant variations in gene expression . Gene pathway analysis revealed consistent variations in gene expression induced by Apis when compared to non-exposed reference cells but not to reference solvent solutions .
2 . Lima LF . Comparison Between the Additive Effects of Diluted ( rFSH ) and Diluted / Dynamized ( FSH 6 cH ) Recombinant Follicle-Stimulating Hormone on the In Vitro Culture of Ovine Preantral Follicles Enclosed in Ovarian Tissue . Compl Ther Med , 2016 , Apr , 25 : 39-44 . This study compared two types of recombinant follicle stimulating hormone ( rFSH ) -diluted and homeopathically potentised , on in-vitro development of ovine follicles . In the first experiment , ovarian fragments were cultured for 1 or 7 days in α-MEM (+) in the absence or presence of different concentrations of diluted rFSH to determine the best concentration . In the second experiment , the effect of diluted and potentised rFSH ( rFSH 6C ), alone or in combination , was studied . In experiment 1 , compared to control , 50ng / mL of diluted rFSH induced higher rates of follicular survival after 7 days of culture and higher percentages of growing follicles at day 1 of culture . In experiment 2 , compared to controls , rFSH 6C induced higher follicular diameter and survival rate after 7 days and early follicle activation at day 1 of culture ( P < 0.05 ). Compared to diluted rFSH , rFSH 6C induced higher rates of follicle activation at day 1 of culture .
3 . Mondal J , Samadder A , Khuda- Bukhsh AR . Psorinum 6X Triggers Apoptosis Signals in Human Lung Cancer Cells . J Integr Med , 2016 , Mar , 14 , 2 , 143-153 . This controlled study was conducted to examine the in-vitro effects of the homeopathic medicine , Psorinum 6X , on cancer cells . Cancer cell lines , including A549 , HepG2 and MCF-7 , were exposed to homeopathically-prepared Psorinum 6X or a control , and the outcomes assessed using a 3- ( 4,5-dimethylthiazol-2-yl ) - 2,5-diphenyltetrazolium bromide assay . The cell line that exhibited the greatest level of inhibition was selected and used in further assays to examine cell cycle arrest , cell death ( apoptosis ), generation of reactive oxygen species ( ROS ) and change in mitochondrial membrane potential ( MMP ), with assessment carried out by flow cytometry and fluorescence microscopy . Expression of several signal proteins related to apoptosis and cell survival was quantified with Western blotting and confocal microscopy . Further , circular dichroism ( CD ) spectroscopy was used to determine possible drug-DNA interactions , as well as the induction of conformational changes . The results showed that Psorinum 6X triggered apoptosis in A549 cells via both upregulation and down-regulation of relevant signalling proteins , including p53 , caspase-3 , Bax and Bcl-2 .
4 . Lee KJ , Yeo MG . Homeopathic Rhus toxicodendron Has Dual Effects on the Inflammatory Response in the Mouse Preosteoblastic Cell Line MC3T3-e1 . Homeopathy , 2016 , 105 , 1 , 42-47 . A preosteoblastic mouse cell line , MC3T3-e1 , was treated with different homeopathic potencies of Rhus tox and COX-2 mRNA and protein expression was examined using reverse transcriptasepolymerase chain reaction ( RT-PCR ) and immunoblotting . Additionally , nitric oxide ( NO ) generation was examined in LPS-induced MC3T3-e1 cells using a Griess reaction assay . The results showed that exposure to different potencies of Rhus tox increased the expression of Cox2 mRNA , with Rhus tox 30X showing the most prominent increase in mRNA expression . In addition , treatment with Rhus tox 30X significantly increased prostaglandin E2 release compared with other homeopathic potencies . NO generation was dramatically decreased in MC3T3-e1 cells after Rhus tox treatment co-stimulated with lipopolysaccharide . Generally , exposure to homeopathicallyprepared Rhus tox was associated with a modulation of inflammation in the cell population that was studied .
30 | vol29 | no1 | JATMS