Anuario Raza Polo Argentino 2016 | Page 271

each of their genes. And for this same reason, the most usual scenario is that each individual should be a combination of the most characteristic traits of their dam and sire. GENES ARE REGULATED BY THE ENVIRONMENT If all this were to work in a simple linear manner, what would happen is that animals with the same genome would be exactly alike. However, as we have seen this year during the high-goal season in Argentina, individuals that are genetically identical, as is the case of clones, could present small phenotype differences. And this occurs because the genes of each horse interact with the external environment which molds the way in which genetic combinations are expressed in its phenotype. In order to explain this situation, we must bear in mind the environment in which the animals have developed, pointing out that when geneticists speak of environment, we are not referring to climate only, but to all external stimuli that may have a bearing on the animal’s development. Among this group of stimuli we include, for example, the way in which it was tamed; the type of training; what nutrition it received; if it underwent diseases or injuries; the climate in which it was raised, and an almost interminable list of small etceteras. And it is also important to emphasize the fact that these interactions include those that the offspring receive during gestation and during those first years in which animals are most sensitive. Even taking into account all these factors, we still cannot reach a round figure, because it has been determined that adding all the effects of an individual’s genome and the environment that surrounds it, it is still impossible to explain its phenotype by 100%. This is the reason why a few years ago the term “EPIGENETICS” was coined (“epi” means “above”), to describe DNA alterations that occur without changing the nucleotide sequence. This new section of genetics is what is used to try and close the circle that would explain the mechanisms that “mold gene expression” so as to produce a certain phenotype. EPIGENETIC REGULATION If we go back to classic genetic theory, presumably no phenotype change that has been produced by the environment is heritable by its descendants. This is so because these observable changes were not produced by an alteration in the genetic sequence of the horse, but by the way in which the environment caused this genome to express itself. For example, a foal that has been underfed as it was growing up, and has therefore grown less than expected, should not necessarily produce smaller offspring because its DNA has not undergone any change. In order to understand this we must know that the horse’s genome is expressed through a process in which the DNA is read in each cell and turned into proteins in charge of producing the rest of the molecules that make up the organism. Control of this reading process is given by cellular mechanisms that enable or make difficult the access to the machine that reads DNA strands in which the genetic codes that shall regulate the life of the animal are found. Thus, when a coded protein in a gene is needed for a metabolic process as, for example, bone growth in a colt during its development, these regulating factors make reading of certain specific genes possible, allowing an increase in the amount of bone cells produced. Once development has concluded, these growth genes are “silenced” and are no longer used by the cells of the adult horse. Likewise, the expression or inhibition of certain genes is the cause of having such different cells, such as a neuron or a muscle fiber, even though all of them maintain identical DNA nucleotide sequences. These processes are known as “epigenetic regulation”. One of the most important epigenetic mechanisms is DNA METHYLATION, which consists of a chemical mark added to specific regions of the genome, which make them less accessible and therefore diminish the expression of these genes, to the point of even being able to silence them completely. Therefore, depending on what tissue we are dealing with, or the age of the animal, or external 269